PMID:24151908

Citation	Liu, C, Sun, Z, Shen, S, Lin, L, Li, T, Tian, B and Hua, Y (2014) Identification and characterization of the geranylgeranyl diphosphate synthase in Deinococcus radiodurans. Lett. Appl. Microbiol. 58:219-24
Abstract	Deinococcus radiodurans strain R1 utilizes multiple antioxidants including a unique carotenoid, deinoxanthin, to fight again oxidative stress. Most of the enzymes involved in the deinoxanthin biosynthetic pathway have been identified. However, the enzyme catalysing the synthesis of geranylgeranyl diphosphate (GGPP), which is a precursor of carotenoid biosynthesis, has yet to be identified. Two putative isoprenyl diphosphate synthases (IPPS) homologues (DR1395 and DR932) were screened out by analysis of conserved amino acid regions, and their biochemical functions were investigated. Gene mutation, gene expression in Escherichia coli and analysis of carotenoid products were used to investigate the functions of these candidates. The results suggested that DR1395 encodes the protein for GGPP synthesis. Site-directed mutant analysis indicated that the amino acid composition of and around the first aspartate-rich motif is vital for GGPP synthase function.
Links	PubMed Online version:10.1111/lam.12181
Keywords
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Significance

Gene product	Qualifier	GO Term	Evidence Code	Aspect	Notes	Status
DEIRA:Q9RUJ1	involved_in	GO:0008299: isoprenoid biosynthetic process	ECO:0000315: mutant phenotype evidence used in manual assertion	P	Seeded From UniProt	complete
DEIRA:Q9RUJ1		GO:0008299: isoprenoid biosynthetic process	ECO:0000315:	P	Figure 3. Shows the absorbance of E.coli cotransformed with plasmids carrying wild-type (BI-95) or mutant dr932 genes; absorbance indicates the production of the red carotenoid. The results show that plasmids containing mutant dr932 genes do not produce the carotenoid but the wildtype BI-95 does.	complete CACAO 9870

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