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ARATH:HEN1
Contents
| Species (Taxon ID) | Arabidopsis thaliana (Mouse-ear cress). (3702) | |
| Gene Name(s) | HEN1 (synonyms: CRM2) | |
| Protein Name(s) | Small RNA 2'-O-methyltransferase
Protein CORYMBOSA 2 Protein HUA ENHANCER 1 S-adenosylmethionine-dependent RNA methyltransferase HEN1 | |
| External Links | ||
| UniProt | Q9C5Q8 | |
| EMBL | AF060248 AF411383 AF327068 AL080282 AL161553 CP002687 CP002687 | |
| PIR | T10633 | |
| RefSeq | NP_001190782.1 NP_567616.1 | |
| UniGene | At.3574 | |
| PDB | 3HTX | |
| PDBsum | 3HTX | |
| ProteinModelPortal | Q9C5Q8 | |
| SMR | Q9C5Q8 | |
| BioGrid | 13130 | |
| STRING | 3702.AT4G20910.1 | |
| iPTMnet | Q9C5Q8 | |
| PaxDb | Q9C5Q8 | |
| PRIDE | Q9C5Q8 | |
| EnsemblPlants | AT4G20910.1 AT4G20910.2 | |
| GeneID | 827839 | |
| Gramene | AT4G20910.1 AT4G20910.2 | |
| KEGG | ath:AT4G20910 | |
| TAIR | AT4G20910 | |
| eggNOG | KOG1045 ENOG410XSD6 | |
| HOGENOM | HOG000084447 | |
| OMA | SANCESS | |
| PhylomeDB | Q9C5Q8 | |
| EvolutionaryTrace | Q9C5Q8 | |
| PRO | PR:Q9C5Q8 | |
| Proteomes | UP000006548 | |
| Genevisible | Q9C5Q8 | |
| GO | GO:0005737 GO:0005634 GO:0046872 GO:0008171 GO:0003723 GO:0008173 GO:0010589 GO:0010305 GO:0035279 GO:0035196 GO:0030422 GO:0009909 GO:0010093 GO:0009616 | |
| Gene3D | 3.40.50.150 | |
| InterPro | IPR026610 IPR006630 IPR025714 IPR029063 | |
| PANTHER | PTHR21404 | |
| Pfam | PF13847 | |
| SUPFAM | SSF53335 | |
| PROSITE | PS50961 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
involved_in |
GO:0035279 |
mRNA cleavage involved in gene silencing by miRNA |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0035196 |
production of miRNAs involved in gene silencing by miRNA |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010589 |
leaf proximal/distal pattern formation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010305 |
leaf vascular tissue pattern formation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0010093 |
specification of floral organ identity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009909 |
regulation of flower development |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0009616 |
virus induced gene silencing |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008173 |
RNA methyltransferase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008173 |
RNA methyltransferase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0034587 |
piRNA metabolic process |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0033686 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0030422 |
production of siRNA involved in RNA interference |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0033686 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008173 |
RNA methyltransferase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:1913965 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0008171 |
O-methyltransferase activity |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0033686 |
F |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000483941 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
PANTHER:PTN000483941 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0001510 |
RNA methylation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008171 |
O-methyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008173 |
RNA methyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032259 |
methylation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0031047 |
gene silencing by RNA |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008168 |
methyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0061715 |
miRNA 2'-O-methylation |
ECO:0000314 |
P |
Figures 1 and 2 show that HEN1 methylates miRNA duplexes. Figure 3 shows that the position of methylation is the 2’OH of the 3’ terminal nucleotide. |
complete | |||||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Allen, E et al. (2005) microRNA-directed phasing during trans-acting siRNA biogenesis in plants. Cell 121 207-21 PubMed GONUTS page
- ↑ Boutet, S et al. (2003) Arabidopsis HEN1: a genetic link between endogenous miRNA controlling development and siRNA controlling transgene silencing and virus resistance. Curr. Biol. 13 843-8 PubMed GONUTS page
- ↑ 3.0 3.1 Jover-Gil, S et al. (2012) The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis. Plant Cell Physiol. 53 1322-33 PubMed GONUTS page
- ↑ Chen, X et al. (2002) HEN1 functions pleiotropically in Arabidopsis development and acts in C function in the flower. Development 129 1085-94 PubMed GONUTS page
- ↑ Suzuki, M et al. (2002) Formation of corymb-like inflorescences due to delay in bolting and flower development in the corymbosa2 mutant of Arabidopsis. Plant Cell Physiol. 43 298-306 PubMed GONUTS page
- ↑ Blevins, T et al. (2006) Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Res. 34 6233-46 PubMed GONUTS page
- ↑ Li, J et al. (2005) Methylation protects miRNAs and siRNAs from a 3'-end uridylation activity in Arabidopsis. Curr. Biol. 15 1501-7 PubMed GONUTS page
- ↑ Yu, B et al. (2005) Methylation as a crucial step in plant microRNA biogenesis. Science 307 932-5 PubMed GONUTS page
- ↑ 9.0 9.1 Fang, Y & Spector, DL (2007) Identification of nuclear dicing bodies containing proteins for microRNA biogenesis in living Arabidopsis plants. Curr. Biol. 17 818-23 PubMed GONUTS page
- ↑ 10.0 10.1 10.2 10.3 10.4 10.5 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ Yang, Z et al. (2006) HEN1 recognizes 21-24 nt small RNA duplexes and deposits a methyl group onto the 2' OH of the 3' terminal nucleotide. Nucleic Acids Res. 34 667-75 PubMed GONUTS page
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