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PMID:15705854
| Citation |
Yu, B, Yang, Z, Li, J, Minakhina, S, Yang, M, Padgett, RW, Steward, R and Chen, X (2005) Methylation as a crucial step in plant microRNA biogenesis. Science 307:932-5 |
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| Abstract |
Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2'-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs. |
| Links |
PubMed Online version:10.1126/science.1107130 |
| Keywords |
Arabidopsis/metabolism; Arabidopsis Proteins/metabolism; Mass Spectrometry; Methylation; MicroRNAs/chemistry; MicroRNAs/metabolism; Oligonucleotides, Antisense; RNA, Plant/chemistry; RNA, Plant/metabolism; RNA, Small Interfering/chemistry; RNA, Small Interfering/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Ribose/metabolism; Substrate Specificity |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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