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YEAST:SET1
Contents
| Species (Taxon ID) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). (559292) | |
| Gene Name(s) | SET1 (synonyms: KMT2, YTX1) | |
| Protein Name(s) | Histone-lysine N-methyltransferase, H3 lysine-4 specific
COMPASS component SET1 Lysine N-methyltransferase 2 SET domain-containing protein 1 | |
| External Links | ||
| UniProt | P38827 | |
| EMBL | U00059 BK006934 | |
| PIR | S48961 | |
| RefSeq | NP_011987.1 | |
| PDB | 2J8A | |
| PDBsum | 2J8A | |
| ProteinModelPortal | P38827 | |
| SMR | P38827 | |
| BioGrid | 36552 | |
| DIP | DIP-4616N | |
| IntAct | P38827 | |
| MINT | MINT-552558 | |
| STRING | 4932.YHR119W | |
| MaxQB | P38827 | |
| PaxDb | P38827 | |
| PRIDE | P38827 | |
| EnsemblFungi | [example_ID YHR119W] | |
| GeneID | 856519 | |
| KEGG | sce:YHR119W | |
| CYGD | YHR119w | |
| SGD | S000001161 | |
| eggNOG | COG2940 | |
| GeneTree | ENSGT00760000119228 | |
| HOGENOM | HOG000066111 | |
| InParanoid | P38827 | |
| KO | K11422 | |
| OMA | ERLPCLC | |
| OrthoDB | EOG7P8PH3 | |
| BioCyc | YEAST:G3O-31161-MONOMER | |
| EvolutionaryTrace | P38827 | |
| NextBio | 982275 | |
| PRO | PR:P38827 | |
| Proteomes | UP000002311 | |
| Genevestigator | P38827 | |
| GO | GO:0005694 GO:0048188 GO:0042054 GO:0018024 GO:0016279 GO:0003723 GO:0030437 GO:0000183 GO:0030466 GO:0006348 GO:0051568 GO:0018027 GO:0035066 GO:0043618 GO:0000723 | |
| InterPro | IPR024657 IPR017111 IPR003616 IPR024636 IPR001214 | |
| Pfam | PF11764 PF00856 PF11767 | |
| PIRSF | PIRSF037104 | |
| SMART | SM00508 SM00317 | |
| PROSITE | PS50868 PS51572 PS50280 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0080182 |
histone H3-K4 trimethylation |
ECO:0000315 |
P |
Figure 3. Shows a Western blot, in which it shows wild-type SET1 has H3K4 trimethylating abilities. |
complete | |||||
| GO:0044648 |
histone H3-K4 dimethylation |
ECO:0000315 |
P |
The Western Blot in Figure 3. Shows wild-type SET1 has H3K4 dimethylating abilities. |
complete | |||||
| GO:0080182 |
histone H3-K4 trimethylation |
ECO:0000315 |
P |
Figure 1B and Figure 3A both show wild type SET1 (labeled as FL Set1 for "Full-length Set1"), is involved in H3K4 trimethylation. |
complete | |||||
| GO:0044648 |
histone H3-K4 dimethylation |
ECO:0000315 |
P |
Both Figure 1B and Figure 3A show Set1 (labeled as FL set1 for "Full length Set1") is involved in dimethylation. |
complete | |||||
| GO:0035961 |
positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter |
ECO:0000315 |
P |
Figure 2 |
complete | |||||
| GO:0055092 |
sterol homeostasis |
ECO:0000315 |
P |
Figure 4 |
complete | |||||
| GO:1990872 |
negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter |
ECO:0000315 |
P |
Figure 5 |
complete | |||||
| GO:0070452 |
positive regulation of ergosterol biosynthetic process |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
|
complete | ||||
| GO:0051568 |
histone H3-K4 methylation |
ECO:0000315 |
P |
Figure3A and C |
complete | |||||
| GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
F |
Figure 3A and C |
complete | |||||
| GO:0044648 |
histone H3-K4 dimethylation |
ECO:0000315 |
P |
Figure 4C |
complete | |||||
| GO:0080182 |
histone H3-K4 trimethylation |
ECO:0000315 |
P |
Figure 4C |
complete | |||||
| GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
F |
Figure 4C |
complete | |||||
| GO:1905088 |
positive regulation of synaptonemal complex assembly |
ECO:0000315 |
P |
The set1 single mutant shows clear defects in SC assembly (Figure 5A and 5B). |
complete | |||||
| GO:0007130 |
synaptonemal complex assembly |
ECO:0000315 |
P |
Figure 5A illustrates set1 contribute to formation of synaptonemal complex between Hop1 and Zip1. |
complete | |||||
| GO:0097692 |
histone H3-K4 monomethylation |
ECO:0000315 |
P |
Fig. 3 shows that S. cerevisiae cells with the wild type set1 gene produce monomethylated H3K4 complexes while some of the amino acid substitution mutants do not produces these monomethylated complexes. |
complete | |||||
| GO:0000183 |
chromatin silencing at rDNA |
ECO:0000315 |
P |
Fig. 4 shows that S. cerevisiae containing the set1 gene (labeled SET1+::ura3-52) silences rDNA transcription while the mutant lacking the set1 gene does not. |
complete | |||||
| GO:0006479 |
protein methylation |
ECO:0000315 |
P |
Fig. 5 shows that S. cerevisiae containing a functional set1 gene (labeled SET1+) demonstrates limited growth at 30 degrees Celsius, while the colonies lacking the set1 gene show more growth at 30 degrees Celsius. Limited growth at 30 degrees indicates that methylation has not occured, while increased growth indicates a lack of methylation. “Deletion of SET1 suppresses the temperature-sensitive growth defect of cells carrying a conditional allele of the Aurora kinase, ipl1-2. Previous work has shown that this suppression is due to reduced methylation of Dam1 in the absence of Set1 [41].” |
complete | |||||
| GO:0010629 |
negative regulation of gene expression |
ECO:0000315 |
P |
Fig 1. Shows that in S. cerevisiae, the set1 gene is required for repressing the expression of other S. cerevisiae middle class sporulation genes during vegetative growth. |
complete | |||||
| GO:0090241 |
negative regulation of histone H4 acetylation |
ECO:0000315 |
P |
Fig. 5 shows that in S. cerevisiae lacking the set1 gene, H3K5 acetylation increases at middle sporulation loci. |
complete | |||||
|
part_of |
GO:0000781 |
chromosome, telomeric region |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006348 |
chromatin silencing at telomere |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1902275 |
regulation of chromatin organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1903341 |
regulation of meiotic DNA double-strand break formation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044648 |
histone H3-K4 dimethylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0080182 |
histone H3-K4 trimethylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0044648 |
histone H3-K4 dimethylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0080182 |
histone H3-K4 trimethylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051568 |
histone H3-K4 methylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051568 |
histone H3-K4 methylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051568 |
histone H3-K4 methylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051568 |
histone H3-K4 methylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051568 |
histone H3-K4 methylation |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0048188 |
Set1C/COMPASS complex |
ECO:0000353 |
physical interaction evidence used in manual assertion |
SGD:S000000064 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0048188 |
Set1C/COMPASS complex |
ECO:0000353 |
physical interaction evidence used in manual assertion |
SGD:S000000064 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0048188 |
Set1C/COMPASS complex |
ECO:0000353 |
physical interaction evidence used in manual assertion |
SGD:S000000064 |
C |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0043618 |
regulation of transcription from RNA polymerase II promoter in response to stress |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
SGD:S000006074 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0043618 |
regulation of transcription from RNA polymerase II promoter in response to stress |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
contributes_to |
GO:0042800 |
histone methyltransferase activity (H3-K4 specific) |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0042054 |
histone methyltransferase activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
Missing: with/from | |||
|
involved_in |
GO:0035066 |
positive regulation of histone acetylation |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
SGD:S000003704 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0030466 |
chromatin silencing at silent mating-type cassette |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0030437 |
ascospore formation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0018027 |
peptidyl-lysine dimethylation |
ECO:0000353 |
physical interaction evidence used in manual assertion |
SGD:S000003345 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0018027 |
peptidyl-lysine dimethylation |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016279 |
protein-lysine N-methyltransferase activity |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
SGD:S000003345 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0016279 |
protein-lysine N-methyltransferase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006348 |
chromatin silencing at telomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006348 |
chromatin silencing at telomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006348 |
chromatin silencing at telomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006348 |
chromatin silencing at telomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000723 |
telomere maintenance |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0000723 |
telomere maintenance |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0018024 |
histone-lysine N-methyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0034968 |
histone lysine methylation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0048188 |
Set1C/COMPASS complex |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0018024 |
histone-lysine N-methyltransferase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006325 |
chromatin organization |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032259 |
methylation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005694 |
chromosome |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008168 |
methyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Williamson, K et al. (2013) Catalytic and functional roles of conserved amino acids in the SET domain of the S. cerevisiae lysine methyltransferase Set1. PLoS ONE 8 e57974 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 Thornton, JL et al. (2014) Context dependency of Set1/COMPASS-mediated histone H3 Lys4 trimethylation. Genes Dev. 28 115-20 PubMed GONUTS page
- ↑ 3.0 3.1 3.2 3.3 South, PF et al. (2013) H3K4 methyltransferase Set1 is involved in maintenance of ergosterol homeostasis and resistance to Brefeldin A. Proc. Natl. Acad. Sci. U.S.A. 110 E1016-25 PubMed GONUTS page
- ↑ 4.0 4.1 Briggs, SD et al. (2001) Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae. Genes Dev. 15 3286-95 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 Takahashi, YH et al. (2011) Structural analysis of the core COMPASS family of histone H3K4 methylases from yeast to human. Proc. Natl. Acad. Sci. U.S.A. 108 20526-31 PubMed GONUTS page
- ↑ 6.0 6.1 Bani Ismail, M et al. (2014) Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast. PLoS ONE 9 e96648 PubMed GONUTS page
- ↑ 7.0 7.1 Jaiswal, D et al. (2017) Repression of Middle Sporulation Genes in by the Sum1-Rfm1-Hst1 Complex Is Maintained by Set1 and H3K4 Methylation. G3 (Bethesda) 7 3971-3982 PubMed GONUTS page
- ↑ 8.0 8.1 Laible, G et al. (1997) Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres. EMBO J. 16 3219-32 PubMed GONUTS page
- ↑ 9.0 9.1 9.2 Ramakrishnan, S et al. (2016) Counteracting H3K4 methylation modulators Set1 and Jhd2 co-regulate chromatin dynamics and gene transcription. Nat Commun 7 11949 PubMed GONUTS page
- ↑ Sommermeyer, V et al. (2013) Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes. Mol. Cell 49 43-54 PubMed GONUTS page
- ↑ 11.0 11.1 11.2 11.3 11.4 Nagy, PL et al. (2002) A trithorax-group complex purified from Saccharomyces cerevisiae is required for methylation of histone H3. Proc. Natl. Acad. Sci. U.S.A. 99 90-4 PubMed GONUTS page
- ↑ 12.0 12.1 12.2 12.3 12.4 Krogan, NJ et al. (2002) COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. J. Biol. Chem. 277 10753-5 PubMed GONUTS page
- ↑ 13.0 13.1 13.2 13.3 13.4 Roguev, A et al. (2001) The Saccharomyces cerevisiae Set1 complex includes an Ash2 homologue and methylates histone 3 lysine 4. EMBO J. 20 7137-48 PubMed GONUTS page
- ↑ Miller, T et al. (2001) COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Proc. Natl. Acad. Sci. U.S.A. 98 12902-7 PubMed GONUTS page
- ↑ 15.0 15.1 Seol, JH et al. (2006) Different roles of histone H3 lysine 4 methylation in chromatin maintenance. Biochem. Biophys. Res. Commun. 349 463-70 PubMed GONUTS page
- ↑ 16.0 16.1 Boa, S et al. (2003) Saccharomyces cerevisiae Set1p is a methyltransferase specific for lysine 4 of histone H3 and is required for efficient gene expression. Yeast 20 827-35 PubMed GONUTS page
- ↑ Ginsburg, DS et al. (2009) NuA4 lysine acetyltransferase Esa1 is targeted to coding regions and stimulates transcription elongation with Gcn5. Mol. Cell. Biol. 29 6473-87 PubMed GONUTS page
- ↑ 18.0 18.1 18.2 Nislow, C et al. (1997) SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes. Mol. Biol. Cell 8 2421-36 PubMed GONUTS page
- ↑ Morohashi, N et al. (2005) Effect of histone methyltransferase gene mutations on sporulation in S. cerevisiae. Nucleic Acids Symp Ser (Oxf) 325-6 PubMed GONUTS page
- ↑ 20.0 20.1 20.2 20.3 Zhang, K et al. (2005) The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation. Cell 122 723-34 PubMed GONUTS page
- ↑ Corda, Y et al. (1999) Interaction between Set1p and checkpoint protein Mec3p in DNA repair and telomere functions. Nat. Genet. 21 204-8 PubMed GONUTS page
- ↑ 22.0 22.1 Trésaugues, L et al. (2006) Structural characterization of Set1 RNA recognition motifs and their role in histone H3 lysine 4 methylation. J. Mol. Biol. 359 1170-81 PubMed GONUTS page
c
d
f
h
n
o
- GO:0044648 ! obsolete histone H3-K4 dimethylation
- GO:0097692 ! obsolete histone H3-K4 monomethylation
- GO:0051568 ! obsolete histone H3-K4 methylation
- GO:0034968 ! obsolete histone lysine methylation
- GO:0018024 ! obsolete histone lysine N-methyltransferase activity
- GO:0080182 ! obsolete histone H3-K4 trimethylation
- GO:0090241 ! obsolete negative regulation of histone H4 acetylation
- GO:1990872 ! obsolete negative regulation of sterol import by negative regulation of transcription from RNA polymerase II promoter
- GO:0035066 ! obsolete positive regulation of histone acetylation
- GO:0035961 ! obsolete positive regulation of ergosterol biosynthetic process by positive regulation of transcription from RNA polymerase II promoter
- GO:0043618 ! obsolete regulation of transcription from RNA polymerase II promoter in response to stress
p
- GO:0018027 ! peptidyl-lysine dimethylation
- GO:1905088 ! positive regulation of synaptonemal complex assembly
- GO:0045944 ! positive regulation of transcription by RNA polymerase II
- GO:0070452 ! positive regulation of ergosterol biosynthetic process
- GO:0016279 ! protein-lysine N-methyltransferase activity
- GO:0006479 ! protein methylation
r
s
