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PMID:16143104
| Citation |
Zhang, K, Lin, W, Latham, JA, Riefler, GM, Schumacher, JM, Chan, C, Tatchell, K, Hawke, DH, Kobayashi, R and Dent, SY (2005) The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation. Cell 122:723-34 |
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| Abstract |
A balance in the activities of the Ipl Aurora kinase and the Glc7 phosphatase is essential for normal chromosome segregation in yeast. We report here that this balance is modulated by the Set1 methyltransferase. Deletion of SET1 suppresses chromosome loss in ipl1-2 cells. Conversely, combination of SET1 and GLC7 mutations is lethal. Strikingly, these effects are independent of previously defined functions for Set1 in transcription initiation and histone H3 methylation. We find that Set1 is required for methylation of conserved lysines in a kinetochore protein, Dam1. Biochemical and genetic experiments indicate that Dam1 methylation inhibits Ipl1-mediated phosphorylation of flanking serines. Our studies demonstrate that Set1 has important, unexpected functions in mitosis. Moreover, our findings suggest that antagonism between lysine methylation and serine phosphorylation is a fundamental mechanism for controlling protein function. |
| Links |
PubMed PMC1794220 Online version:10.1016/j.cell.2005.06.021 |
| Keywords |
Amino Acid Sequence; Cell Cycle Proteins/metabolism; Chromosome Deletion; Chromosome Segregation/genetics; Chromosome Segregation/physiology; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology; Gene Deletion; Histone-Lysine N-Methyltransferase; Intracellular Signaling Peptides and Proteins; Methylation; Methyltransferases/genetics; Methyltransferases/physiology; Microtubule-Associated Proteins/metabolism; Mitosis/genetics; Mitosis/physiology; Molecular Sequence Data; Mutation; Phenotype; Phosphorylation; Protein Kinases/genetics; Protein Kinases/physiology; Protein-Serine-Threonine Kinases; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae Proteins/genetics; Saccharomyces cerevisiae Proteins/metabolism; Saccharomyces cerevisiae Proteins/physiology; Sequence Alignment; Transcription Factors/genetics; Transcription Factors/physiology |
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