User:Tsingh3

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	CATRO:Q1WMA5	2013-09-15 17:58:48 CDT	GO:0008172 S-methyltransferase activity (F)	PMID:16623883	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 Used radioactive S-adenosyl-L-[methyl-3H] methionine and quantified product via radioactivity (Tritium labelled.	challenge
unacceptable	MICEC:Q8KND7	2013-09-29 12:41:40 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:18721755	ECO:0000314 direct assay evidence used in manual assertion	Glycosyltransferase CalG3 is from organism Micromonospora echinospora, derived from the calicheamicin gene cluster. Structure is solved by X-ray crystallography (Fig 4).	challenge
updatedbyinstructor	MICEC:Q8KND7	2013-09-29 12:44:06 CDT	GO:0046527 glucosyltransferase activity (F)	PMID:18721755	ECO:0000314 direct assay evidence used in manual assertion	Glycosyltransferase CalG3 is derived from a calicheamicin gene cluster from the organism Micromonospora echinospora.Its function is characterized by HPLC (High Pressure Liquid Chromatography) and Mass spectrometry (Fig 3 & 5).	challenge
unacceptable	MICEC:Q8KNE0	2013-09-29 12:53:48 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:21987796	ECO:0000314 direct assay evidence used in manual assertion	CalG2 is a glycosyltransferase from Micromonospora echinospora derived from the calicheamicin gene cluster. Structure of CalG2 was solved by X-ray crystallography. Fig 2 & 3 show the structure with bound substrate and the catalytic residues.	challenge
requireschanges	MICEC:Q8KNE0	2013-09-29 13:00:46 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:18721755	ECO:0000314 direct assay evidence used in manual assertion	CalG2 is a glycosyltransferase from the calicheamicin gene cluster in the organism, Micromonospora echinospora.Function was characterized by HPLC (High Pressure Liquid Chromatography) and Mass spectrometry as shown in Fig 5.	challenge
unacceptable	MICEC:Q8KNF2	2013-09-29 13:21:05 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:21987796	ECO:0000314 direct assay evidence used in manual assertion	CalG1 is a glycosyltransferase in the calicheamicin gene cluster found in the Micromonospora echinospora organism. Its structure is solved by X-ray crystallography, as shown in Fig 2D & 3D.	challenge
requireschanges	MICEC:Q8KNF2	2013-09-29 13:29:12 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:16946071	ECO:0000314 direct assay evidence used in manual assertion	CalG1 is a glycosyltransferase from the calicheamicin gene cluster in the organism, Micromonospora echinospora. Its function is characterized via HPLC and Mass spectrometry (Fig 1).	challenge
unacceptable	MICEC:Q8KNC3	2013-10-26 16:42:00 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:21987796	ECO:0000314 direct assay evidence used in manual assertion	The structure of the Glycosyltransferase isolated from Micromonospora echinospora via X-ray crystallography. The gene for the Glycosyltransferase is located on the Calicheamicin gene cluster.Structure is shown in Fig 2C and 3C.	challenge
unacceptable	MICEC:Q8KNF1	2013-10-26 17:10:13 CDT		PMID:23662776	ECO:0000314 direct assay evidence used in manual assertion	CalS11 is a sugar O-methyltransferase from calicheamicin gene cluster from the organism Micromonospora echinospora.Its structure was discovered in this paper, as seen in Figures 4-7.	challenge
acceptable	MICEC:Q8KNF1	2013-10-26 17:10:14 CDT	GO:0008171 O-methyltransferase activity (F)	PMID:23662776	ECO:0000314 direct assay evidence used in manual assertion	The function of CalG4 was determined in this paper via NMR spectroscopy as shown in Figure 3.	challenge
unacceptable	MICEC:Q8KNC3	2013-10-26 17:18:38 CDT	GO:0016757 transferase activity, transferring glycosyl groups (F)	PMID:18721755	ECO:0000314 direct assay evidence used in manual assertion	Function of CalG4 was characterized by HPLC (in the reversible step between step 7 & 1), in Figure 6.	challenge
unacceptable	?	2013-10-26 17:29:53 CDT		PMID:21538548	IDA: Inferred from Direct Assay		challenge
updatedbyinstructor	STRLA:MMCR	2013-10-26 17:48:54 CDT	GO:0008171 O-methyltransferase activity (F)	PMID:17461583	ECO:0000314 direct assay evidence used in manual assertion	MmcR is a Hydroxyquinone O-Methyltransferase from mitomycin gene cluster in Streptomyces lavendulae. Its function is determined here by various methods such as liquid chromatography, TLC, and assays with radiolabeling (Figure 2, 3, & 4).	challenge

acceptable:2
unacceptable:7
requires_changes:2
flagged:0

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