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User:Swans181

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableTRYBB:O762902013-04-13 20:19:14 CDTGO:0047040 pteridine reductase activity (F)PMID:20545846ECO:0000315 mutant phenotype evidence used in manual assertion

Using figures 2 and 3 to determine the function for PTR1. Figure 2 shows the production of tetracycline with and without PTR1 in the cells and their resulting virulence in Figure 4. Figure 3 shows the enzyme activity of PTR1.

challenge
updatedbyinstructorTRYB2:Q4GZ502013-04-13 20:35:47 CDTGO:0004525 ribonuclease III activity (F)PMID:16963561ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows U-deletion cleavage activity of rTbMP90 (also known as KREPB1). Deletion of this is used, mutating the trypanosomes and changing the phenotype as a result.

challenge
requireschanges9TRYP:P906292013-04-13 21:00:31 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:109187ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows an immunoprecipitation, demonstrating that without gBP21, the RNA no longer has separation from editing.

challenge

acceptable:1
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Swans181

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Swans181

What do the icons mean in the status column?

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