User:Smehr1

Phage Hunters Spring 2017

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	BPP22:CAPSD	2017-04-17 10:24:06 CDT	GO:0046797 viral procapsid maturation (P)	PMID:26269173	ECO:0000315 mutant phenotype evidence used in manual assertion	Mutant phenotypes D244A and D246A were used to show the role of the D-loop in procapsid maturation. In figure 2A, the wild type shows 2 peaks corresponding to procapsid protein around fraction 16 and mature phages that incorporated the coat protein at fraction 23. D244A mutant showed improperly formed procapsids in figure 2B which could correspond to the protein peak at fraction 16 in figure 2A. D244A showed very little coat protein at fraction 23 indicating phages were not produced. The D246A mutants showed no significant amount of procapsid protein at fraction 16. In figure 2B the D246A mutant showed particles that were incomplete instead of procapsid proteins that were present in wild types. This mutant also showed no peak of mature coat protein at fraction 23 indicating the phages were not able to form.	challenge

acceptable:1
unacceptable:0
requires_changes:0
flagged:0

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Lma2, Team Blue-B	BPPH2:NP	GO:0017053 - transcription repressor complex (C)	PMID:12426390	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1

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