Phage Hunters Spring 2017
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | BPPH2:NP | 2017-04-08 15:22:49 CDT | GO:0017053 transcriptional repressor complex (C) | PMID:12426390 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 2B. A DNase I footprinting assay with both the wild type and mutant was run to observe the p6 and p4 complexes with a DNA fragment containing promoters A3, A2b, and A2c. Calles et al. incubated each lane with an increasing concentration of p6 or p4 and observed the footprints, or areas where the DNase fails to cut because of complex bounded onto the DNA. Mutant p4 proteins lead to a decrease in repression due to the failure of the p4-p6 nucleocomplex protein to form, showing the importance of the p4 protein in transcriptional repression. The presence of p4 protein increases the affinity of the p6 protein to bind, which would repress transcription.
| challenge |
| updatedbyinstructor | BPPH2:NP | 2017-04-19 10:26:11 CDT | GO:0017053 transcriptional repressor complex (C) | PMID:12426390 | ECO:0000314 direct assay evidence used in manual assertion | Figure 3. Competitive EMSA with increasing amounts of [p4] and [p6] incubated in each lane to observe the p4-p6 nucleoprotein complex formation at the late A3 promoter between positions -58 to -104. P4 by itself binds downstream of -58 position; P6 by itself binds throughout the -58 to -104 position; suggesting that the p4 protein allows specific sites for the complex to form.
| challenge |
acceptable:1
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by Lma2
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by Lma2
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