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Cacao

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GO:0090569cytoplasmic transcriptional repressor complexPMID:12426390IDA: Inferred from Direct Assay C
This annotation made on page: BPPH2:NP
By: Lma2 (group Team Blue-B) on 2017-04-08 15:22:49 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeSmehr1,
Team Purple-B
2017-04-12 10:10:10 CDT

Figure shows the comparison of wild type to mutants, IDA should be IMP.

10
Private
Assessment
Ivanerill2017-05-25 04:57:43 CDTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
Ivanerill2017-05-24 06:25:49 CDT

Evidence and GO term addressed, but WITH field still missing

Requires Changes
Go term
Evidence
With/From
Public
Assessment
DanielRenfro2017-04-19 10:12:06 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0017053 transcriptional repressor complex PMID:12426390 IMP: Inferred from Mutant Phenotype C Figure 2B. A DNase I footprinting assay with both the wild type and mutant was run to observe the p6 and p4 complexes with a DNA fragment containing promoters A3, A2b, and A2c. Calles et al. incubated each lane with an increasing concentration of p6 or p4 and observed the footprints, or areas where the DNase fails to cut because of complex bounded onto the DNA. Mutant p4 proteins lead to a decrease in repression due to the failure of the p4-p6 nucleocomplex protein to form, showing the importance of the p4 protein in transcriptional repression. The presence of p4 protein increases the affinity of the p6 protein to bind, which would repress transcription. complete
CACAO 12503
on BPPH2:NP
Flagged
Public
Assessment
Ivanerill2017-04-15 17:19:29 CDT

Evidence should be IMP. Why cytoplasmic trans. repr. complex here and not just trans. repr. complex as in other annotations? experiment is in vitro. protein complex annotation should include other partner id in WITH

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original