requireschanges | COFAR:XMT1 | 2012-04-09 15:51:15 CDT | GO:0008170 N-methyltransferase activity (F) | PMID:12746542 | ECO:0000314 direct assay evidence used in manual assertion | Based on methods: three types of cDNAs encoding N-methyltransferases were isolated from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. The bacterially expressed encoded proteins were characterized for their catalytic properties. Using these recombinant proteins, catalytic specificity was determined with the following substrates: XMP, xanthosine, xanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, paraxanthine, theobromine, and theophylline (Fig. (Fig.3B–D;3B–D; Table TableI).I). Purified CaXMT1 solely catalyzed conversion of xanthosine to 7-methylxanthosine
| challenge |
requireschanges | COFAR:MXMT1 | 2012-04-09 17:30:55 CDT | GO:0008170 N-methyltransferase activity (F) | PMID:12746542 | ECO:0000314 direct assay evidence used in manual assertion | three types of cDNAs encoding N-methyltransferases were isolated from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. The bacterially expressed encoded proteins were characterized for their catalytic properties. Using these recombinant proteins, catalytic specificity was determined with the following substrates: XMP, xanthosine, xanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, paraxanthine, theobromine, and theophylline (Fig. (Fig.3B–D;3B–D; Table TableI).I). Purified CaXMT1 solely catalyzed conversion of xanthosine to 7-methylxanthosine (Fig. (Fig.3B,3B, lane 2). In contrast, crude CaXMT1 catalyzed conversion of xanthosine to 7-methylxanthine, suggesting that conversion of xanthosine to 7-methylxanthosine and that of 7-methylxanthosine to 7-methylxanthine successively occurred (Fig. (Fig.3B,3B, lane 4). Although the former reaction was catalyzed by CaMXT1, the latter was found to be catalyzed by a nonspecific purine-nucleoside phosphorylase derived from E. coli (Fig. (Fig.3B,3B, lane 6).
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requireschanges | COFAR:MXMT2 | 2012-04-09 17:35:07 CDT | GO:0008170 N-methyltransferase activity (F) | PMID:12746542 | ECO:0000314 direct assay evidence used in manual assertion | three types of cDNAs encoding N-methyltransferases were isolated from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. The bacterially expressed encoded proteins were characterized for their catalytic properties. Using these recombinant proteins, catalytic specificity was determined with the following substrates: XMP, xanthosine, xanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, paraxanthine, theobromine, and theophylline (Fig. (Fig.3B–D;3B–D; Table TableI).I). CaMXMT2 catalyzed conversion of 7-methylxanthine to theobromine (Fig. (Fig.3C,3C, lane 2).
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acceptable | ARATH:HAL3A | 2012-04-15 14:57:40 CDT | GO:0015937 coenzyme A biosynthetic process (P) | PMID:16415216 | ECO:0000315 mutant phenotype evidence used in manual assertion | Based on Fig. 5 and 6; Total fatty acid and acyl-CoA measurements of 5-d-old aaBb (null for HAL3A and heterozygous for HAL3B) seedlings in medium lacking Suc revealed stalled storage lipid catabolism and impaired CoA biosynthesis; in particular, acetyl-CoA levels were reduced by approximately 80%. Taken together, these results provide in vivo evidence for the function of HAL3A and HAL3B, and they point out the critical role of CoA biosynthesis during early postgerminative growth.
| challenge |
updatedbyinstructor | STRVZ:Q5U913 | 2012-04-15 16:45:43 CDT | GO:0016491 oxidoreductase activity (F) | PMID:15817470 | ECO:0000314 direct assay evidence used in manual assertion | Based on table 1; for JadH, both UWM6 and 2,3-dehydro-UWM6 underwent 4a,12b-dehydration and C-12 monooxygenation and were converted to rabelomycin and dehydrorabelomycin, respectively. Rabelomycin remained unchanged. 3) In controls without supplemented enzymes, the substrates remained unchanged.
| challenge |
updatedbyinstructor | STRVZ:Q5U913 | 2012-04-15 16:47:27 CDT | GO:0016836 hydro-lyase activity (F) | PMID:15817470 | ECO:0000314 direct assay evidence used in manual assertion | Based on table 1: for JadH, both UWM6 and 2,3-dehydro-UWM6 underwent 4a,12b-dehydration and C-12 monooxygenation and were converted to rabelomycin and dehydrorabelomycin, respectively. Rabelomycin remained unchanged. 3) In controls without supplemented enzymes, the substrates remained unchanged.
| challenge |
updatedbyinstructor | RHOER:MLHB | 2012-04-15 17:29:57 CDT | GO:0046573 lactonohydrolase activity (F) | PMID:11157238 | ECO:0000314 direct assay evidence used in manual assertion | | challenge |