COFAR:MXMT1

Species (Taxon ID)	Coffea arabica (Arabian coffee). (13443)
Gene Name(s)	MXMT1 (synonyms: CTS1)
Protein Name(s)	Monomethylxanthine methyltransferase 1 CaMXMT1 Theobromine synthase 1
External Links
UniProt	Q9AVJ9
EMBL	AB048794 AB034700 AF494411
ProteinModelPortal	Q9AVJ9
SMR	Q9AVJ9
BRENDA	2.1.1.159
GO	GO:0005737 GO:0008168 GO:0009820
Gene3D	3.40.50.150
InterPro	IPR005299 IPR029063
Pfam	PF03492
SUPFAM	SSF53335

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0008170	N-methyltransferase activity	PMID:12746542	ECO:0000314			F		three types of cDNAs encoding N-methyltransferases were isolated from immature fruits of coffee (Coffea arabica) plants, and designated as CaXMT1, CaMXMT2, and CaDXMT1, respectively. The bacterially expressed encoded proteins were characterized for their catalytic properties. Using these recombinant proteins, catalytic specificity was determined with the following substrates: XMP, xanthosine, xanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, paraxanthine, theobromine, and theophylline (Fig. ​(Fig.3B–D;3B–D; Table ​TableI).I). Purified CaXMT1 solely catalyzed conversion of xanthosine to 7-methylxanthosine (Fig. ​(Fig.3B,3B, lane 2). In contrast, crude CaXMT1 catalyzed conversion of xanthosine to 7-methylxanthine, suggesting that conversion of xanthosine to 7-methylxanthosine and that of 7-methylxanthosine to 7-methylxanthine successively occurred (Fig. ​(Fig.3B,3B, lane 4). Although the former reaction was catalyzed by CaMXT1, the latter was found to be catalyzed by a nonspecific purine-nucleoside phosphorylase derived from E. coli (Fig. ​(Fig.3B,3B, lane 6).	complete CACAO 4255
enables	GO:0008168	methyltransferase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR005299	F		Seeded From UniProt	complete
involved_in	GO:0009820	alkaloid metabolic process	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0017	P		Seeded From UniProt	complete
part_of	GO:0005737	cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0963 UniProtKB-SubCell:SL-0086	C		Seeded From UniProt	complete
involved_in	GO:0032259	methylation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0489	P		Seeded From UniProt	complete
enables	GO:0016740	transferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0808	F		Seeded From UniProt	complete
enables	GO:0008168	methyltransferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0489	F		Seeded From UniProt	complete
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References

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