User:Me094014

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	STRMU:SUCP	2012-03-26 14:24:14 CDT	GO:0005985 sucrose metabolic process (P)	PMID:18402606	ECO:0000314 direct assay evidence used in manual assertion	(Table 1)biofilms of S. mutans grown with starch in combination with sucrose exhibited biomass in production of the biofilm.	challenge
requireschanges	STRMU:SUCP	2012-03-26 14:26:08 CDT	GO:0005975 carbohydrate metabolic process (P)	PMID:18402606	ECO:0000314 direct assay evidence used in manual assertion	Figure 2.   Amylase activity indicated by the amount of reducing sugars released into the medium during incubation of sHA in 1% starch. The amylase activities at 2, 4, and 6 h after salivary pellicle formation were not significantly different from each other. he presence of sucrose alone or in combinations resulted in biofilms displaying four to seven times more biomass, seven to 15 times more total protein, and 10 to 15 times more total EPS than starch or starch + glucose + fructose-grown biofilms (Table 1).	challenge
acceptable	ECOLI:ENVZ	2012-04-14 12:30:37 CDT	GO:0046777 protein autophosphorylation (P)	PMID:10973966	ECO:0000315 mutant phenotype evidence used in manual assertion	EnvZ mutants cannot properly autophosphorylate in Fig 3.	challenge
requireschanges	ECOLI:ENVZ	2012-04-14 12:44:07 CDT	GO:0016301 kinase activity (F)	PMID:10973966	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 and Figure 5. Phosphotransfer from phosphorylated EnvZc and EnvZc(T247X) mutant proteins to OmpR.A. EnvZ-P, phosphorylated EnvZ.	challenge
requireschanges	ECOLI:ENVZ	2012-04-14 13:24:34 CDT	GO:0006470 protein dephosphorylation (P)	PMID:2656684	ECO:0000314 direct assay evidence used in manual assertion	FIG. 7. Autoradiograms showing the time course of dephosphorylation of EnvZ* mediated by a substoichiometric amount of OmpR.	challenge
requireschanges	ECOLI:ENVZ	2012-04-14 14:07:29 CDT	GO:0071229 cellular response to acid (P)	PMID:20380141	ECO:0000315 mutant phenotype evidence used in manual assertion	Figures 1-5 shows growth rates,and correlation, as the PH increased, survival times for wild type decreased. ensZ mutants survived better than other mutants.	challenge
requireschanges	ECOLI:ENVZ	2012-04-14 15:05:50 CDT	GO:0004721 phosphoprotein phosphatase activity (F)	PMID:10973966	ECO:0000315 mutant phenotype evidence used in manual assertion	figure 7 shows phosphatase activity of mutants relative to wild type	challenge

acceptable:1
unacceptable:0
requires_changes:6
flagged:0

Annotations challenged by Me094014

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Me094014

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links

acceptable:0
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Me094014

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by Me094014