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Category:Team Alfred Hershey

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesECOLI:ENVZMe094014, Team Alfred Hershey2012-04-14 15:05:50 CDTGO:0004721 phosphoprotein phosphatase activity (F)PMID:10973966ECO:0000315 mutant phenotype evidence used in manual assertion

figure 7 shows phosphatase activity of mutants relative to wild type

challenge
requireschangesECOLI:ENVZMe094014, Team Alfred Hershey2012-04-14 14:07:29 CDTGO:0071229 cellular response to acid (P)PMID:20380141ECO:0000315 mutant phenotype evidence used in manual assertion

Figures 1-5 shows growth rates,and correlation, as the PH increased, survival times for wild type decreased. ensZ mutants survived better than other mutants.

challenge
requireschangesECOLI:ENVZMe094014, Team Alfred Hershey2012-04-14 13:24:34 CDTGO:0006470 protein dephosphorylation (P)PMID:2656684ECO:0000314 direct assay evidence used in manual assertion

FIG. 7. Autoradiograms showing the time course of dephosphorylation

of EnvZ* mediated by a substoichiometric amount of OmpR.

challenge
requireschangesECOLI:ENVZMe094014, Team Alfred Hershey2012-04-14 12:44:07 CDTGO:0016301 kinase activity (F)PMID:10973966ECO:0000314 direct assay evidence used in manual assertion

Figure 4 and Figure 5. Phosphotransfer from phosphorylated EnvZc and EnvZc(T247X) mutant proteins to OmpR.A. EnvZ-P, phosphorylated EnvZ.


challenge
acceptableECOLI:ENVZMe094014, Team Alfred Hershey2012-04-14 12:30:37 CDTGO:0046777 protein autophosphorylation (P)PMID:10973966ECO:0000315 mutant phenotype evidence used in manual assertion

EnvZ mutants cannot properly autophosphorylate in Fig 3.

challenge
requireschangesFRATM:B2SEL0Br924723, Team Alfred Hershey2012-04-11 21:19:23 CDTGO:0005975 carbohydrate metabolic process (Figure 2 shows that ΔsigB colonies are not able to grow as strong or have the clearing capabilities of WT and other present mutants)PMID:20675445IMP: Inferred from Mutant Phenotype
challenge
requireschangesFRATM:B2SEL0Br924723, Team Alfred Hershey2012-04-11 21:04:31 CDTGO:0003824 catalytic activity (Figure 4 shows kinetic activity of chiA, chiB, and chiC and a comparative graph)PMID:21531796IDA: Inferred from Direct Assay
challenge
requireschangesSERMA:Q54276Br924723, Team Alfred Hershey2012-04-11 20:52:53 CDTGO:0005576 extracellular region (C)PMID:78947030000002ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows minute extracellular activity using two separate techniques and also in figure 4d with immunogold labeling in S. marcescens BJL200

challenge
requireschangesASPFM:Q873X9Br924723, Team Alfred Hershey2012-04-11 19:59:14 CDTGO:0004568 chitinase activity (F)PMID:14523125ECO:0000269 experimental evidence used in manual assertion

Figure 5c; extracellular chitinase activity

challenge
requireschangesSTRMU:SUCPMe094014, Team Alfred Hershey2012-03-26 14:26:08 CDTGO:0005975 carbohydrate metabolic process (P)PMID:18402606ECO:0000314 direct assay evidence used in manual assertion

Figure 2.   Amylase activity indicated by the amount of reducing sugars released into the medium during incubation of sHA in 1% starch. The amylase activities at 2, 4, and 6 h after salivary pellicle formation were not significantly different from each other. he presence of sucrose alone or in combinations resulted in biofilms displaying four to seven times more biomass, seven to 15 times more total protein, and 10 to 15 times more total EPS than starch or starch + glucose + fructose-grown biofilms (Table 1).

challenge
requireschangesSTRMU:SUCPMe094014, Team Alfred Hershey2012-03-26 14:24:14 CDTGO:0005985 sucrose metabolic process (P)PMID:18402606ECO:0000314 direct assay evidence used in manual assertion

(Table 1)biofilms of S. mutans grown with starch in combination with sucrose exhibited biomass in production of the biofilm.

challenge

Pages in category "Team Alfred Hershey"

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