GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

PMID:2656684

From GONUTS
Jump to: navigation, search
Citation

Aiba, H, Mizuno, T and Mizushima, S (1989) Transfer of phosphoryl group between two regulatory proteins involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli. J. Biol. Chem. 264:8563-7

Abstract

EnvZ is a cytoplasmic membrane protein which is involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli possibly by sensing the environmental osmotic signal. A truncated form of the EnvZ protein (EnvZ*), comprising 82% of EnvZ starting from the C terminus, was purified to homogeneity. The purified EnvZ* was autophosphorylated with ATP. The phosphoryl group on EnvZ* could then be rapidly transferred to OmpR, which is a positive regulator of the ompF and ompC genes and which was proposed to interact with EnvZ in the process of osmoregulation. In the presence of ATP, the phosphorylated OmpR was rapidly dephosphorylated. These results suggest that the transfer of the phosphoryl group between EnvZ and OmpR plays an important role in the signaling pathway in osmoregulation.

Links

PubMed

Keywords

Adenosine Triphosphate/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Genes; Genes, Bacterial; Kinetics; Osmolar Concentration; Phosphorylation; Plasmids

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

ECOLI:ENVZ

GO:0006470: protein dephosphorylation

ECO:0000314:

P

FIG. 7. Autoradiograms showing the time course of dephosphorylation of EnvZ* mediated by a substoichiometric amount of OmpR.

complete
CACAO 4660


See also

References

See Help:References for how to manage references in GONUTS.