GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:2656684
Citation |
Aiba, H, Mizuno, T and Mizushima, S (1989) Transfer of phosphoryl group between two regulatory proteins involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli. J. Biol. Chem. 264:8563-7 |
---|---|
Abstract |
EnvZ is a cytoplasmic membrane protein which is involved in osmoregulatory expression of the ompF and ompC genes in Escherichia coli possibly by sensing the environmental osmotic signal. A truncated form of the EnvZ protein (EnvZ*), comprising 82% of EnvZ starting from the C terminus, was purified to homogeneity. The purified EnvZ* was autophosphorylated with ATP. The phosphoryl group on EnvZ* could then be rapidly transferred to OmpR, which is a positive regulator of the ompF and ompC genes and which was proposed to interact with EnvZ in the process of osmoregulation. In the presence of ATP, the phosphorylated OmpR was rapidly dephosphorylated. These results suggest that the transfer of the phosphoryl group between EnvZ and OmpR plays an important role in the signaling pathway in osmoregulation. |
Links | |
Keywords |
Adenosine Triphosphate/metabolism; Bacterial Outer Membrane Proteins/genetics; Bacterial Outer Membrane Proteins/isolation & purification; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Genes; Genes, Bacterial; Kinetics; Osmolar Concentration; Phosphorylation; Plasmids |
edit table |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0006470: protein dephosphorylation |
ECO:0000314: |
P |
FIG. 7. Autoradiograms showing the time course of dephosphorylation of EnvZ* mediated by a substoichiometric amount of OmpR. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.