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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableECOLX:A0A0E1L6G72017-03-26 09:33:02 CDTGO:0009405 pathogenesis (P)PMID:26965678ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5

Survival assay. One-day-old broiler chicks showed higher survival rates when infected with Escherichia coli (SEPT362) mutant ∆ychO compared to the E.coli (SEPT362) WT strain

updatedbyinstructorECOK1:A0A0H2Z3A52017-04-05 11:31:20 CDTGO:0044650 adhesion of symbiont to host cell (P)PMID:25487442ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3. Quantification of the adhesion of Avian Pathogenic Escherichia coli (Strain: SCI-07 strain) nirC (major nitrite transporter) mutant, complemented or wild-type strains showed lower initial mortality rate; indicative of delayed or decreased pathogenicity.

updatedbyinstructorPSEAI:Q9RLJ12017-04-07 11:24:24 CDTGO:0009405 pathogenesis (P)PMID:22666466ECO:0000316 genetic interaction evidence used in manual assertion

Pseudomonas aeruginosa (PA01 Strain) czcR

"Figure 4 CzcR is required for the full virulence of P. aeruginosa."

" In our assay, an infection with the wild type strain killed 50% of the population within circa 3 days of contact with bacteria and all worms were dead after 4 days (Fig. 4). In contrast, the virulence of the ΔczcRS mutant was significantly reduced since 50% of the worms were still alive after 5 days of contact. The virulence of the ΔczcRS mutant strain complemented with czcR on the pRWT plasmid was partially restored since 50% of the worms were dead after 4 days and less than 20% of the worm population was alive after 5 days, while no complementation was observed with the czcS gene (Fig. 4).



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