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User:MHAoufi

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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableFOAMV:GAG2017-04-20 12:40:53 CDTGO:0039715 nuclear viral factory (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

challenge
unacceptableFOAMV:GAG2017-04-20 12:47:14 CDTGO:0044094 host cell nuclear part (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

challenge
updatedbyinstructorSFVCP:GAG2017-04-20 12:49:56 CDTGO:0042025 host cell nucleus (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

GAG_SFVCP for Simian foamy virus (isolate chimpanzee) (SFVcpz)

challenge
unacceptableFOAMV:GAG2017-04-20 12:58:34 CDTGO:0005737 cytoplasm (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAG2017-04-20 13:01:14 CDTGO:0044444 cytoplasmic part (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAG2017-04-20 13:02:37 CDTGO:0033655 host cell cytoplasm part (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAG2017-04-20 13:04:15 CDTGO:0000787 cytoplasmic nucleosome (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
updatedbyinstructorSFVCP:GAG2017-04-20 13:05:57 CDTGO:0042025 host cell nucleus (C)PMID:21255441ECO:0000315 mutant phenotype evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

The prototype primate foamy virus (PFV), originally designated as human foamy virus (HFV), then as simian foamy virus-chimpanzee (human isolate) [SFVcpz(hu)], is a chimpanzee FV isolated from a human-derived cell culture (Lochelt et al., 1991).

challenge

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unacceptable:6
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Annotations challenged by MHAoufi

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