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Category:Team Integreats

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorSFVCP:GAGXGoh, Team Integreats2017-04-23 16:03:14 CDTGO:0044383 host chromosome (C)PMID:17530924ECO:0000314 direct assay evidence used in manual assertion

Fig 1A. Incoming Gag proteins which were stained with gamma-tubulin antibodies were detected at the MTOC by confocal microscopy and indirect immunofluorescence at 3, 15, and 30 d p.i.

Simian foamy virus (isolate chimpanzee) (SFVcpz)

challenge
unacceptableHUMAN:TOE1FJalih, Team Integreats2017-04-23 15:11:47 CDTGO:0015030 Cajal body (C)PMID:26056259ECO:0000314 direct assay evidence used in manual assertion

FIGURE 4.B. Localization in HeLa cells 1 h after administration of recombinant EGFP-TOE1 fusion protein. Fusion protein involved a 6×His tag and were purified on a nickel column and administered to cultures of HeLa cells at a 1 μM concentration. CellMask Deep-Red plasma membrane stain was added immediately before live imaging at 0.25 μg/mL.

challenge
updatedbyinstructorSFVCP:GAGFJalih, Team Integreats2017-04-23 15:03:34 CDTGO:0042025 host cell cytoplasm (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableHUMAN:TOE1FJalih, Team Integreats2017-04-23 15:01:25 CDTGO:0072495 host cell Cajal body (C)PMID:26056259ECO:0000314 direct assay evidence used in manual assertion

FIGURE 4.B. Localization in HeLa cells 1 h after administration of recombinant EGFP-TOE1 fusion protein. Fusion protein involved a 6×His tag and were purified on a nickel column and administered to cultures of HeLa cells at a 1 μM concentration. CellMask Deep-Red plasma membrane stain was added immediately before live imaging at 0.25 μg/mL.

challenge
updatedbyinstructorSFVCP:GAGMHAoufi, Team Integreats2017-04-20 13:05:57 CDTGO:0042025 host cell nucleus (C)PMID:21255441ECO:0000315 mutant phenotype evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

The prototype primate foamy virus (PFV), originally designated as human foamy virus (HFV), then as simian foamy virus-chimpanzee (human isolate) [SFVcpz(hu)], is a chimpanzee FV isolated from a human-derived cell culture (Lochelt et al., 1991).

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 13:04:15 CDTGO:0000787 cytoplasmic nucleosome (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 13:02:37 CDTGO:0033655 host cell cytoplasm part (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 13:01:14 CDTGO:0044444 cytoplasmic part (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 12:58:34 CDTGO:0005737 cytoplasm (C)PMID:21255441ECO:0000314 direct assay evidence used in manual assertion

Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.

challenge
updatedbyinstructorSFVCP:GAGMHAoufi, Team Integreats2017-04-20 12:49:56 CDTGO:0042025 host cell nucleus (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

GAG_SFVCP for Simian foamy virus (isolate chimpanzee) (SFVcpz)

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 12:47:14 CDTGO:0044094 host cell nuclear part (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

challenge
unacceptableFOAMV:GAGMHAoufi, Team Integreats2017-04-20 12:40:53 CDTGO:0039715 nuclear viral factory (C)PMID:22393357ECO:0000314 direct assay evidence used in manual assertion

Figure1.C. After methanol fixation, cells that were expressing GFP or GFP-GagGRII were examined for intrinsic fluorescence. Nuclei are stained with DAPI. Original magnification ×40. Red arrows indicate a cell undergoing mitosis

challenge
updatedbyinstructorHUMAN:PLK1XGoh, Team Integreats2017-04-07 10:07:05 CDTGO:0005813 centrosome (C)PMID:27579920ECO:0000314 direct assay evidence used in manual assertion

Fig 3D The protein localization patterns of human PLK foci were observed and presumed to be in centrosomes after forty-eight hours, using confocal laser scanning microscopy.

challenge
updatedbyinstructorHUMAN:ZN363XGoh, Team Integreats2017-04-06 15:48:39 CDTGO:0005634 nucleus (C)PMID:25848801ECO:0000314 direct assay evidence used in manual assertion

Figure 1A shows the staining of the nucleus through co-localization via DAPI staining.

RING finger and CHY zinc finger domain-containing protein 1/RCHY1-Homo sapiens

challenge

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Pages in category "Team Integreats"

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