GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

User:Kvh5314

From GONUTS
Jump to: navigation, search

CACAO Fall 2015

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableLISMO:LNTA2015-11-23 16:26:26 CSTGO:0009405 pathogenesis (P)PMID:24449750ECO:0000314 direct assay evidence used in manual assertion

Fig 1.

challenge
updatedbyinstructorLISMO:LNTA2015-11-23 16:45:51 CSTGO:0042025 host cell nucleus (C)PMID:24449750ECO:0000314 direct assay evidence used in manual assertion

Figure S3A - LntA-V5 colocalizes with DAPI, a protein known to localize on the nucleus.

challenge
updatedbyinstructorBACSU:DESK2015-11-24 12:37:41 CSTGO:0004672 protein kinase activity (F)PMID:19805278ECO:0000314 direct assay evidence used in manual assertion

Fig. 4A

The left image shows kinase activity at time intervals at both 25C (top) and 37C (bottom). Kinase activity was up-regulated at lower temperature

challenge
acceptableBACSU:DESK2015-11-24 12:37:41 CSTGO:0004721 phosphoprotein phosphatase activity (F)PMID:19805278ECO:0000314 direct assay evidence used in manual assertion

Fig. 4B

Left figure show phosphatase activity of desK at 25C (top) and 37C (bottom).

challenge
unacceptableBACSU:DESK2015-11-24 12:37:42 CSTGO:0016740 transferase activity (F)PMID:19805278ECO:0000314 direct assay evidence used in manual assertion

Fig. 4C

Figure on the left show phosphotransferase activity of desK at 25C (top) and 37C (bottom).

challenge
unacceptableBACSU:COTH2015-11-24 16:07:38 CSTGO:0030435 sporulation resulting in formation of a cellular spore (P)PMID:25115591ECO:0000314 direct assay evidence used in manual assertion

Figure 5.

in 5A, the ER220 mutant is lacking in only cotH, while still having cotG and cotS present. Figure 5B shows fluorescence microscopy and the ER220 mutant shows fluorescence signal around forming spores but not around mature, free spores. This supports the necessity of cotH in forming mature spores that can germinate efficiently.

challenge
unacceptableBACSU:DEGU2015-11-24 17:56:59 CSTGO:0000160 phosphorelay signal transduction system (P)PMID:21736639ECO:0000314 direct assay evidence used in manual assertion

Figure 3.

Shows degU phosphorylation activates PflgM

challenge

acceptable:1
unacceptable:4
requires_changes:0
flagged:0

Annotations challenged by Kvh5314

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Kvh5314

What do the icons mean in the status column?

assessment icons.jpg