PMID:25115591

Citation	Saggese, A, Scamardella, V, Sirec, T, Cangiano, G, Isticato, R, Pane, F, Amoresano, A, Ricca, E and Baccigalupi, L (2014) Antagonistic role of CotG and CotH on spore germination and coat formation in Bacillus subtilis. PLoS ONE 9:e104900
Abstract	Spore formers are bacteria able to survive harsh environmental conditions by differentiating a specialized, highly resistant spore. In Bacillus subtilis, the model system for spore formers, the recently discovered crust and the proteinaceous coat are the external layers that surround the spore and contribute to its survival. The coat is formed by about seventy different proteins assembled and organized into three layers by the action of a subset of regulatory proteins, referred to as morphogenetic factors. CotH is a morphogenetic factor needed for the development of spores able to germinate efficiently and involved in the assembly of nine outer coat proteins, including CotG. Here we report that CotG has negative effects on spore germination and on the assembly of at least three outer coat proteins. Such negative action is exerted only in mutants lacking CotH, thus suggesting an antagonistic effect of the two proteins, with CotH counteracting the negative role of CotG.
Links	PubMed PMC4130616 Online version:10.1371/journal.pone.0104900
Keywords
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Significance

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:COTH		GO:0030435: sporulation resulting in formation of a cellular spore	ECO:0000314:		P		Figure 5. in 5A, the ER220 mutant is lacking in only cotH, while still having cotG and cotS present. Figure 5B shows fluorescence microscopy and the ER220 mutant shows fluorescence signal around forming spores but not around mature, free spores. This supports the necessity of cotH in forming mature spores that can germinate efficiently.	complete CACAO 11203

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