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User:Krbrown6

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableGLUDA:Q288C92013-09-15 15:08:02 CDTGO:0097460 ferrous iron import into cell (P)PMID:23624722ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows growth of G. diazotrophicus Pal5 and isogenic mutant AS (disrupted feuABC operon) in both iron-present and iron-deficient conditions. Inactivation of FeuABC affected the ability to multiply under iron-restricted conditions, indicating that feuABC is needed for iron uptake.

challenge
updatedbyinstructorVIBF1:Q5DYR12013-09-25 19:41:52 CDTGO:0035885 exochitinase activity (F)PMID:22233679ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8A. The ΔrpoQ mutant showed chitinase activity that was significantly lower than the wild type. Both the wild type and the ΔrpoQ mutant overexpressing RpoQ showed chitinase activity that was 2.3- to 2.8-fold-higher.

challenge
updatedbyinstructorMOUSE:HSP722013-10-08 20:47:22 CDTGO:1901896 positive regulation of calcium-transporting ATPase activity (P)PMID:22495301ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
acceptableRHOS4:Q3IXP72013-10-23 11:39:47 CDTGO:0030494 bacteriochlorophyll biosynthetic process (P)PMID:23252506ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2: R. sphaeroides rsp_3070 was able to restore synthesis of the WT (wild-type) C8-ethyl chlorophyll a.

challenge
unacceptableFRATL:Q7X1682013-11-06 12:05:47 CSTGO:0002437 inflammatory response to antigenic stimulus (P)PMID:19233475ECO:0000314 direct assay evidence used in manual assertion

Figure 6: Tul4 was recognized during acute infections - examined the F. tularensis specific IFNγ responses of mice after intradermal inoculation with tularensis LVS. Splenocytes were isolated and stimulated in vitro for 18–24 h with Tul4 86–99 peptide. Cells were then stained using an array of cellular markers followed by analysis via multicolor flow cytometry, which allowed for identification of activated IFNγ positive CD4+ T cells.

challenge

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