Phage Hunters Spring 2016
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| acceptable | BPMS2:COAT | 2016-04-21 15:25:41 CDT | GO:1904972 negative regulation of viral translation (P) | PMID:4903176 | ECO:0000314 direct assay evidence used in manual assertion | Incubation of MS2 coat protein with RNA showed significantly decreased phage protein production through 14C-labeled proteins and radioautography. The coat protein was shown to have specific inhibitory effect on its homologous RNA, and formed a complex when bound to the RNA. Fig. 5 shows that formation of this complex strongly correlates to inhibitory effect.
| challenge |
| updatedbyinstructor | BPPHS:H | 2016-04-26 14:49:57 CDT | GO:0039638 lipopolysaccharide-mediated virion attachment to host cell (P) | PMID:1094682 | ECO:0000315 mutant phenotype evidence used in manual assertion | Table 2 shows that anti-H antibody inhibits infectivity of the phiX174 SS complex with gene H protein. Figure 3 and Table 3 demonstrate that the Gene H protein is recognized specifically by the phage LPS receptor previously identified. This is evidence implicating gene H in LPS-binding and phage adsorption.
| challenge |
| updatedbyinstructor | BPKMV:HOLIN | 2016-04-25 13:28:15 CDT | GO:0034292 pinholin activity (F) | PMID:21687532 | ECO:0000316 genetic interaction evidence used in manual assertion | Figure 4 shows results of a holin complementation assay. This gene was inserted with lysis cassettes missing the holin gene to confirm holin function. When inserted with the lambda cassette (lambda endolysin), abrupt lysis pattern was not seen. However, lysis is accelerated with expression of this holin gene and a SAR endolysin, suggesting pinholin activity rather than canonical holin activity.
| challenge |
| acceptable | BPMU:CAPSD | 2016-04-25 13:01:02 CDT | GO:0098017 viral capsid, major subunit (C) | PMID:6213090 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1 pinpoints the major capsid protein through 2-dimensional gel electrophoresis. Figure 2 displays gel electrophoretic analysis of extracts produced by lysogens containing amber mutant prophages. T mutants were the only late gene mutants which lacked the major capsid protein, suggesting it is the T gene that encodes for this protein.
| challenge |
| acceptable | LAMBD:DECO | 2016-05-01 22:58:47 CDT | GO:0098021 viral capsid, decoration (C) | PMID:609100 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 10 shows that gene D protein, known to be present in the phage head, does not affect head size, suggesting it provides head stability instead. This is verified in Figure 11, which shows that gene D mutants are more susceptible to EDTA inactivation through the rupturing of the phage head. Protein D is likely a decorator protein as it stabilizes the head but is not essential in the gene D mutants isolated (Figure 2).
| challenge |
acceptable:3
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by JenniferTran
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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