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Eggen, K and Nathans, D (1969) Regulation of protein synthesis directed by coliphage MS2 RNA. II. In vitro repression by phage coat protein. J. Mol. Biol. 39:293-305
No abstract in PubMed
Autoradiography; Binding Sites; Carbon Isotopes; Cell-Free System; Coliphages; Densitometry; Enzyme Repression; Escherichia coli; Histidine/metabolism; Kinetics; Ligases/metabolism; RNA, Messenger/metabolism; Ribonucleases; Threonine/metabolism; Viral Proteins/biosynthesis; Viral Proteins/physiology
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:1904972: negative regulation of viral translation||
Incubation of MS2 coat protein with RNA showed significantly decreased phage protein production through 14C-labeled proteins and radioautography. The coat protein was shown to have specific inhibitory effect on its homologous RNA, and formed a complex when bound to the RNA. Fig. 5 shows that formation of this complex strongly correlates to inhibitory effect.
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