GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:21687532
| Citation |
Briers, Y, Peeters, LM, Volckaert, G and Lavigne, R (2011) The lysis cassette of bacteriophage ϕKMV encodes a signal-arrest-release endolysin and a pinholin. Bacteriophage 1:25-30 |
|---|---|
| Abstract |
The lysis cassette of Pseudomonas aeruginosa phage ϕKMV encodes a holin, endolysin, Rz and Rz1 in the canonical order. It has a tight organization with a high degree of overlapping genes and is highly conserved (between 96 and 100% identity at the protein level) among several other members of the "phiKMV-like viruses." The endolysin KMV45 exhibits characteristics as expected for a signal-arrest-release (SAR) endolysin, whereas the holin KMV44 is a typical pinholin. KMV45 is initially secreted as an inactive, membrane-anchored endolysin, which is subsequently released by membrane depolarization driven by the pinholin KMV44. The SAR domain of KMV45 is necessary for its full enzymatic activity, suggesting a refolding of the catalytic cleft upon release from the membrane. The physical proximity of the catalytic glutamic acid residue close to SAR domain suggests an alternative activation mechanism compared to the SAR endolysin of phages P1, ERA103 and 21. Expression of KMV44 leads to a quick cell lysis when paired with SAR endolysin KMV45, but not with the cytoplasmic phage λ endolysin, indicating the membrane depolarizing function of KMV44 rather than the large hole-making function characteristic of classical holins. |
| Links |
PubMed PMC3109451 Online version:10.4161/bact.1.1.14868 |
| Keywords |
|
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0044659: cytolysis by virus of host cell |
ECO:0007699: cell growth assay evidence used in manual assertion |
P |
Fig. 2A shows that induced recombinant KMV45 causes lysis of culture. UniProtKB: Q7Y2C0 (ENLYS_BPKMV) Organism: Pseudomonas phage phiKMV UniProt submitted name: SAR-endolysin |
complete | ||||
| GO:0034292: pinholin activity |
ECO:0000316: |
UniProtKB:P03705
|
F |
Figure 4 shows results of a holin complementation assay. This gene was inserted with lysis cassettes missing the holin gene to confirm holin function. When inserted with the lambda cassette (lambda endolysin), abrupt lysis pattern was not seen. However, lysis is accelerated with expression of this holin gene and a SAR endolysin, suggesting pinholin activity rather than canonical holin activity. |
complete | |||
Notes
See also
References
See Help:References for how to manage references in GONUTS.
