LAMBD:DECO

Species (Taxon ID)	Escherichia phage lambda (Bacteriophage lambda). (10710)
Gene Name(s)	D
Protein Name(s)	Capsid decoration protein Auxiliary protein D Gene product D gpD Major capsid protein D
External Links
UniProt	P03712
EMBL	J02459
PIR	G04333
RefSeq	NP_040586.1
PDB	1C5E 1TCZ 1VD0
PDBsum	1C5E 1TCZ 1VD0
ProteinModelPortal	P03712
SMR	P03712
IntAct	P03712
GeneID	2703529
KEGG	vg:2703529
OrthoDB	VOG090001BA
EvolutionaryTrace	P03712
Proteomes	UP000001711
GO	GO:0030430 GO:0098021 GO:0019076
Gene3D	2.40.300.10
InterPro	IPR004195
Pfam	PF02924
ProDom	PD020518
SUPFAM	SSF51274

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0098021	viral capsid, decoration	PMID:609100	ECO:0000315			C		Figure 10 shows that gene D protein, known to be present in the phage head, does not affect head size, suggesting it provides head stability instead. This is verified in Figure 11, which shows that gene D mutants are more susceptible to EDTA inactivation through the rupturing of the phage head. Protein D is likely a decorator protein as it stabilizes the head but is not essential in the gene D mutants isolated (Figure 2).	complete CACAO 11936
	GO:0098021	viral capsid, decoration	PMID:25053840	ECO:0000314			C		gpD serves as a decoration protein for the capsid and leads to stronger capsid formation. Procapsids were reconstituted in vitro and expanded through 2.5 M urea treatment. Biophysical properties were then assessed through atomic force microscopy. Figure 5 and Table 2 show that addition of gpD leads to an increase in breaking force (0.78 nN from 0.45nN without gpD), and a rescue of the spring constant (0.10 N/m, compared to 0.06 N/m without gpD and the 0.11 N/m of the procapsid). Addition of gpD also reinforces the capsid's stiffness, as shown by the further increase in Young's modulus (E): 0.88 GPa with gpD, 0.57 GPa without gpD, and 0.16 GPa for the procapsid. Based on these measurements, gpD reinforces the capsid, but is not necessary to its formation.	complete CACAO 13110
	GO:0019073	viral DNA genome packaging	PMID:18801370	ECO:0000314	direct assay evidence used in manual assertion		P		Decoration protein gpD contributes to genome packaging in lambda phage. Here, it was evaluated whether gpD is necessary to genome packaging by acting as structural support for the capsid to withstand the high degree of pressure generated. In fig 3A it is shown that addition of gpD of 5uM and above results in a significant increase in the amount of genomic DNA packaged as indicated by the band. 3B is a dose-response curve, showing an increase in genome packaging with increased dose. Fig4 displays the effect of gpD on genome packaging as related to genome length, with no effect on DNA duplexes <37 kb and substantial effect on duplexes >43kb. These results then indicate that gpD reinforces the capsid head such that large DNA duplexes, like the 48kb of lambda phage, can be packaged effectively.	complete CACAO 13200
part_of	GO:0098021	viral capsid, decoration	PMID:18786402	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
part_of	GO:0019028	viral capsid	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0167	C		Seeded From UniProt	complete
part_of	GO:0019012	virion	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0946 UniProtKB-SubCell:SL-0274	C		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C		Seeded From UniProt	complete
part_of	GO:0098021	viral capsid, decoration	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1232	C		Seeded From UniProt	complete
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