User:FJalih

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	SFVCP:GAG	2017-04-23 15:03:34 CDT	GO:0042025 host cell cytoplasm (C)	PMID:21255441	ECO:0000314 direct assay evidence used in manual assertion	Figure1.D. Subcellular localization of GagWT and GagG110V in Hela transfected cells with GagWT or GagG110V and analyzed, 24 h post-transfection, by confocal microscopy following indirect immunofluorescence using rabbit polyclonal anti-PFV. GagWT.	challenge
unacceptable	HUMAN:TOE1	2017-04-23 15:01:25 CDT	GO:0072495 host cell Cajal body (C)	PMID:26056259	ECO:0000314 direct assay evidence used in manual assertion	FIGURE 4.B. Localization in HeLa cells 1 h after administration of recombinant EGFP-TOE1 fusion protein. Fusion protein involved a 6×His tag and were purified on a nickel column and administered to cultures of HeLa cells at a 1 μM concentration. CellMask Deep-Red plasma membrane stain was added immediately before live imaging at 0.25 μg/mL.	challenge
unacceptable	HUMAN:TOE1	2017-04-23 15:11:47 CDT	GO:0015030 Cajal body (C)	PMID:26056259	ECO:0000314 direct assay evidence used in manual assertion	FIGURE 4.B. Localization in HeLa cells 1 h after administration of recombinant EGFP-TOE1 fusion protein. Fusion protein involved a 6×His tag and were purified on a nickel column and administered to cultures of HeLa cells at a 1 μM concentration. CellMask Deep-Red plasma membrane stain was added immediately before live imaging at 0.25 μg/mL.	challenge

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Annotations challenged by FJalih

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