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User:Colganph

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CACAO Spring 2014

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorNEIME:G1FG652014-04-05 17:48:46 CDTGO:0006493 protein O-linked glycosylation (P)PMID:17890310ECO:0000314 direct assay evidence used in manual assertion

Figure 3a shows that E. coli transformed with a plasmid encoding PglL showed a band of lower electrophoretic mobility, which indicates glycosylation of MC pilin, where E. coli not transformed with the plasmid shows no glycosylation.

challenge
unacceptableHUMAN:FABPH2014-04-06 10:49:29 CDTGO:0005324 long-chain fatty acid transporter activity (F)PMID:10567233ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows that mutations in the amino acid sequence of FABH decreases binding affinity for long chain fatty acid oleic acid.

challenge
unacceptableECOLI:RPOS2014-04-06 13:27:03 CDTGO:0001123 transcription initiation from bacterial-type RNA polymerase promoter (P)PMID:21398637ECO:0000314 direct assay evidence used in manual assertion

Table 1 shows that transcription of several genes is initiated by RpoS in the absence of any other sigma factor.

challenge
acceptable9DELT:Q19VP02014-04-06 15:14:41 CDTGO:0008810 cellulase activity (F)PMID:19325747ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows an accumulation of reducing sugars after treating ASC with Cel48 glycosyl hyrdolase

challenge
acceptable9DELT:Q9RAI82014-04-06 15:18:53 CDTGO:0008810 cellulase activity (F)PMID:19325747ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows an accumulation of reducing sugars after treating ASC with Cel9 glycosyl hydrolase

challenge

acceptable:2
unacceptable:2
requires_changes:0
flagged:0

Annotations challenged by Colganph

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0 annotations fixed by Colganph

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