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PMID:19325747
| Citation |
Ramírez-Ramírez, N, Romero-García, ER, Calderón, VC, Avitia, CI, Téllez-Valencia, A and Pedraza-Reyes, M (2008) Expression, characterization and synergistic interactions of Myxobacter Sp. AL-1 Cel9 and Cel48 glycosyl hydrolases. Int J Mol Sci 9:247-57 |
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| Abstract |
The soil microorganism Myxobacter Sp. AL-1 regulates in a differential manner the production of five extracellular cellulases during its life cycle. The nucleotide sequence of a cel9-cel48 cluster from the genome of this microorganism was recently obtained. Cel48 was expressed in Escherichia coli to generate a His(6)-Cel48 protein and the biochemical properties of the pure protein were determined. Cel48 was more efficient in degrading acid-swollen avicel (ASC) than carboxymethylcellulose (CMC). On the other hand, cel9 was expressed in Bacillus subtilis from an IPTG-inducible promoter. Zymogram analysis showed that after IPTG-induction, Cel9 existed in both the cell fraction and the culture medium of B. subtilis and the secreted protein was purified to homogeneity by FPLC-ionic exchange chromatography. The exocellobiohydrolase Cel48 showed a synergism of 1.68 times with the endocellulase Cel9 during ASC degradation using an 8.1-fold excess of Cel48 over Cel9. Western blot analysis revealed that both proteins were synthesized and secreted to the culture medium of Myxobacter Sp. AL-1. These results show that the cel9-cel48 cluster encodes functional endo- and exo-acting cellulases that allows Myobacter Sp. AL-1 to hydrolyse cellulose. |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008810: cellulase activity |
ECO:0000314: |
F |
Figure 5 shows an accumulation of reducing sugars after treating ASC with Cel48 glycosyl hyrdolase |
complete | ||||
|
enables |
GO:0008810: cellulase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
| GO:0008810: cellulase activity |
ECO:0000314: |
F |
Figure 5 shows an accumulation of reducing sugars after treating ASC with Cel9 glycosyl hydrolase |
complete | ||||
|
enables |
GO:0008810: cellulase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
See also
References
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