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User:Awhaas

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableMEDTR:O049982013-09-13 16:51:50 CDTGO:0004356 glutamate-ammonia ligase activity (F)PMID:21914816ECO:0000314 direct assay evidence used in manual assertion

Figure 1 - An assay exhibiting the effects of PN (Figure 1.A) and TNM at pH 8 (Figure 1.B) on glutamine synthetase activity

challenge
requireschangesMOUSE:GAN2013-09-29 16:02:00 CDTGO:0005856 cytoskeleton (C)PMID:23585478ECO:0000315 mutant phenotype evidence used in manual assertion

From figure 2A and 2B, it is shown that the mutant version of gigaxonin, named S52G gigaxonin, was unable to remove vimentin while WT gigaxonin was able to remove it. This proved that gigaxonin was able to affect VIFs but not other cytoskeletal proteins.

challenge
requireschangesHUMAN:TCO22013-10-13 16:55:55 CDTGO:0031419 cobalamin binding (F)PMID:24008152ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1b v shows the effect of the addition of immunostated rabbit, anti-human TCII H-260 to Rhodamine-labelled goat, anti-rabbit IgG secondary antibody on TCII protein expression

challenge
unacceptableECOLX:Q9S4972013-11-10 17:53:25 CSTGO:0007155 cell adhesion (P)PMID:24097079ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6C shows the level of adhesion of FimH at different stress levels of different FimH variants.

challenge

acceptable:1
unacceptable:1
requires_changes:2
flagged:0

Annotations challenged by Awhaas

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
requireschangesDrl5141,
Team Protein Titans
HUMAN:KSYKGO:0006468 - protein phosphorylation (P)PMID:19151749ECO:0000314 direct assay evidence used in manual assertionchallengeC: 4
updatedbyinstructorVthompsonui,
Team Buster McThunderstick
STRMU:Q8DT30GO:0009312 - oligosaccharide biosynthetic process (P)PMID:23930155ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1

fixed by Awhaas

1 annotations fixed by Awhaas

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