User:Alberthi

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	ACRCH:CEFG	2014-04-01 14:00:07 CDT	GO:0033813 deacetylcephalosporin-C acetyltransferase activity (F)	PMID:1569032	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 6 shows that the transformant that did not obtain the cefG gene (371p43) showed a much lower ability to produce cephalosporin from deacetylcephalosporin than the transformants that obtained the cefG gene (371p43.1, 371p43.2, and 371p43.3).	challenge
updatedbyinstructor	XANCB:B0RSR5	2014-04-01 22:38:20 CDT	GO:0003994 aconitate hydratase activity (F)	PMID:9663682	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2 shows a zymogram of the aconitase activity of the wild type in lane one, and the complemented rpfA mutant in lane three.	challenge
updatedbyinstructor	ACRCH:Q8NKG7	2014-04-04 17:16:54 CDT	GO:0042887 amide transmembrane transporter activity (F)	PMID:18668246	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3 parts C and D shows that the penicillin excreted/produced is significantly more in the P. chrysogenum TAT15 and TAT53 transformants carrying the A. chrysogenum cefT gene than the P. chrysogenum T98 strain that did not contain the gene.	challenge
acceptable	PENCH:Q3HUS0	2014-04-05 10:01:50 CDT	GO:0050640 isopenicillin-N N-acyltransferase activity (F)	PMID:1900348	ECO:0000315 mutant phenotype evidence used in manual assertion	In Figure 3, the line with the triangle markers shows the production of benzylpenicillin. In C. acremonium without the penDE gene, there was none produced, but in the transformants with the penDE gene the benzylpenicillin was produced.	challenge

acceptable:2
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Alberthi

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
unacceptable	Wiestcar, MichSt14A 53	ARATH:F4IU18	GO:0032006 - regulation of TOR signaling (Figure 4 shows that when Ncs6p is removed, there is no growth in the presence of rapamycin but when rol5 is added, growth begins again.)	PMID:20530756	IMP: Inferred from Mutant Phenotype	challenge	C: 2
updatedbyinstructor	Alberthi, MichSt14A 25	XANCB:B0RSR5	GO:0003994 - aconitate hydratase activity (F)	PMID:9663682	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 2 fixed by Alberthi
updatedbyinstructor	Michelel, MichSt14A 16	SYNE7:CMPA	GO:0015106 - bicarbonate transmembrane transporter activity (F)	PMID:10557362	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

1 annotations fixed by Alberthi

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