Category:MichSt14A 25

In Figure 3, the line with the triangle markers shows the production of benzylpenicillin. In C. acremonium without the penDE gene, there was none produced, but in the transformants with the penDE gene the benzylpenicillin was produced.

Figure 3 parts C and D shows that the penicillin excreted/produced is significantly more in the P. chrysogenum TAT15 and TAT53 transformants carrying the A. chrysogenum cefT gene than the P. chrysogenum T98 strain that did not contain the gene.

Figure 2 shows a zymogram of the aconitase activity of the wild type in lane one, and the complemented rpfA mutant in lane three.

Figure 6 shows that the transformant that did not obtain the cefG gene (371p43) showed a much lower ability to produce cephalosporin from deacetylcephalosporin than the transformants that obtained the cefG gene (371p43.1, 371p43.2, and 371p43.3).