User:Abulku

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	STRAZ:Q8GGN6	2015-03-30 22:59:46 CDT	GO:1904091 peptidyl carrier protein activity involved in nonribosomal peptide biosynthesis (F)	PMID:17502372	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3 shows the in vitro aminoacylation of holo-LnmP by LnmQ. Fig. 4 shows the aminoacylation of LnmP by LnmQ in trans.	challenge
acceptable	PLAF7:Q8ILW0	2015-03-30 23:24:19 CDT	GO:0016887 ATPase activity (F)	PMID:21722645	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows the ATPase activity of SufC with Km and Kcat values.	challenge
acceptable	PLAF7:Q8ILW0	2015-03-30 23:24:19 CDT	GO:0020011 apicoplast (C)	PMID:21722645	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3A shows the colocalization of SufC with ACP-GFP targeted to the apicoplast. SufC-GFP does not colocalize with the mitocondrion stained with MitoTracker red.	challenge
acceptable	PLAF7:Q25799	2015-03-30 23:34:11 CDT	GO:0016887 ATPase activity (F)	PMID:21722645	ECO:0000314 direct assay evidence used in manual assertion	Fig. 5 shows the ATPase activty of SufB with and without the presence of SufC.	challenge
updatedbyinstructor	9ACTN:Q9XC48	2015-03-31 15:38:40 CDT	GO:1904091 peptidyl carrier protein activity involved in nonribosomal peptide biosynthesis (F)	PMID:10421758	ECO:0000314 direct assay evidence used in manual assertion	Fig. 5 shows the in vivo and in vitro phosphopantetheinylation of BlmI using radiolabelled phosphopantetheine precursor and pantetheine.	challenge
unacceptable	?	2015-03-31 15:38:40 CDT	GO:1904091 peptidyl carrier protein activity involved in nonribosomal peptide biosynthesis (Fig. 7 shows that BlmI can be aminoacylated with valine by an adenylation enzyme not native to the bleomycin NRPS.)	PMID:10421758	IDA: Inferred from Direct Assay		challenge
requireschanges	PSEFL:I4KGG8	2015-03-31 16:31:52 CDT	GO:0042158 lipoprotein biosynthetic process (P)	PMID:25777009	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 2B shows that mutants lacking clpA do not produce massetolide A (lipopeptide) and that complementation of clpA restores production of the lipoprotein. This indicates that ClpA is required for lipopeptide biosynethesis.	challenge
requireschanges	PSEFL:I4K5Z9	2015-03-31 16:27:51 CDT	GO:0042158 lipoprotein biosynthetic process (P)	PMID:19114474	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 1B shows HPLC analysis of massetolide A production of WT and clpP mutants. Strains lacking clpP are unable to produce massetolide A; however, this is massetolide A production is restored upon complementation.	challenge
requireschanges	PSEFL:I4K5Z9	2015-03-31 16:27:51 CDT	GO:0071978 bacterial-type flagellum-dependent swarming motility (P)	PMID:19114474	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 3D shows the swarming motility of WT and clpP mutants. Strains lacking clpP are unable to swarm; however, this is restored by complementation.	challenge
acceptable	PSEFL:I4K5Z9	2015-03-31 16:27:52 CDT	GO:1900192 positive regulation of single-species biofilm formation (P)	PMID:19114474	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 3C shows the biofilm formation of WT and clpP mutants. The ability to form a biofilm in strains lacking clpP is reduced. Biofilm formation was restored upon complementation.	challenge
requireschanges	PSEFL:I4KGG8	2015-03-31 16:31:52 CDT	GO:0071978 bacterial-type flagellum-dependent swarming motility (P)	PMID:25777009	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 2C Shows that clpA mutants are unable to swarm, a process dependent on massetolide A. This is restored with a complement.	challenge

acceptable:5
unacceptable:1
requires_changes:4
flagged:0

Annotations challenged by Abulku

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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