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PMID:21722645

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Citation

Kumar, B, Chaubey, S, Shah, P, Tanveer, A, Charan, M, Siddiqi, MI and Habib, S (2011) Interaction between sulphur mobilisation proteins SufB and SufC: evidence for an iron-sulphur cluster biogenesis pathway in the apicoplast of Plasmodium falciparum. Int. J. Parasitol. 41:991-9

Abstract

The plastid of Plasmodium falciparum, the apicoplast, performs metabolic functions essential to the parasite. Various reactions in the plastid require the assembly of [Fe-S] prosthetic groups on participating proteins as well as the reductant activity of ferredoxin that is converted from its apo-form by the assembly of [Fe-S] clusters inside the apicoplast. The [Fe-S] assembly pathway involving sulphur mobilising Suf proteins has been predicted to function in the apicoplast with one component (PfSufB) encoded by the plastid genome itself. We demonstrate the ATPase activity of recombinant P. falciparum nuclear-encoded SufC and its localisation in the apicoplast. Further, an internal region of apicoplast SufB was used to detect PfSufB-PfSufC interaction in vitro; co-elution of SufB from parasite lysate with recombinant PfSufC on an affinity column also indicated an interaction of the two proteins. As a departure from bacterial SufB and similar to reported plant plastid SufB, apicoplast SufB exhibited ATPase activity, suggesting the evolution of specialised functions in the plastid counterparts. Our results provide experimental evidence for an active Suf pathway in the Plasmodium apicoplast.

Links

PubMed Online version:10.1016/j.ijpara.2011.05.006

Keywords

Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Iron-Sulfur Proteins/genetics; Iron-Sulfur Proteins/metabolism; Molecular Sequence Data; Plasmodium falciparum/enzymology; Plasmodium falciparum/genetics; Plasmodium falciparum/metabolism; Plastids/enzymology; Plastids/genetics; Plastids/metabolism; Protein Binding; Protozoan Proteins/genetics; Protozoan Proteins/metabolism; Sulfur/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PLAF7:Q8ILW0

located_in

GO:0020011: apicoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

PLAF7:Q8ILW0

GO:0016887: ATPase activity

ECO:0000314:

F

Fig. 2 shows the ATPase activity of SufC with Km and Kcat values.

complete
CACAO 10827

PLAF7:Q8ILW0

GO:0020011: apicoplast

ECO:0000314:

C

Fig. 3A shows the colocalization of SufC with ACP-GFP targeted to the apicoplast. SufC-GFP does not colocalize with the mitocondrion stained with MitoTracker red.

complete
CACAO 10828

PLAF7:Q8ILW0

part_of

GO:0020011: apicoplast

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

PLAF7:Q8ILW0

enables

GO:0016887: ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

PLAF7:Q25799

GO:0016887: ATPase activity

ECO:0000314:

F

Fig. 5 shows the ATPase activty of SufB with and without the presence of SufC.

complete
CACAO 10829

PLAF7:Q25799

enables

GO:0016887: ATPase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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