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PMID:10421758
| Citation |
Du, L and Shen, B (1999) Identification and characterization of a type II peptidyl carrier protein from the bleomycin producer Streptomyces verticillus ATCC 15003. Chem. Biol. 6:507-17 |
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| Abstract |
Nonribosomal peptide synthetases (NRPSs) catalyze the assembly of a structurally diverse group of peptides by the multiple-carrier thiotemplate mechanism. All NRPSs known to date are exclusively type I modular enzymes that consist of domains, such as adenylation (A), peptidyl carrier protein (PCP) and condensation (C) domains, for individual enzyme activities. Although several A and PCP domains have been demonstrated to function independently, aminoacylation in trans has been successful only between PCPs and their cognate A domains. |
| Links |
PubMed Online version:10.1016/S1074-5521(99)80083-0 |
| Keywords |
Adenine/metabolism; Amino Acid Sequence; Amino Acids/chemistry; Amino Acids/metabolism; Anti-Bacterial Agents/biosynthesis; Bacterial Proteins; Base Sequence; Bleomycin/biosynthesis; Carrier Proteins/biosynthesis; Carrier Proteins/chemistry; Carrier Proteins/genetics; Chromatography, High Pressure Liquid; Cloning, Molecular; DNA, Fungal/genetics; DNA, Fungal/isolation & purification; Escherichia coli/metabolism; Genes, Fungal/genetics; Mass Spectrometry; Molecular Sequence Data; Streptomyces/genetics; Streptomyces/metabolism; Transfection; Transferases (Other Substituted Phosphate Groups)/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:1904091: peptidyl carrier protein activity involved in nonribosomal peptide biosynthesis |
ECO:0000314: |
F |
Fig. 5 shows the in vivo and in vitro phosphopantetheinylation of BlmI using radiolabelled phosphopantetheine precursor and pantetheine. |
complete | ||||
|
enables |
GO:1904091: peptidyl carrier protein activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
Notes
See also
References
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