GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0008174 | mRNA methyltransferase activity | PMID:8610444 | IMP: Inferred from Mutant Phenotype | F | ||||
| This annotation made on page: SINDV:POLN By: Aaron.Bedford (group Team Just Name It Something) on 2014-03-22 19:15:01 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Aaron.Bedford, Team Just Name It Something | 2014-04-10 13:50:51 CDT | The Fig. 1 (Wang, O'Rear, Stollar) of Mutagenesis of the Sinbis nsP1 Protein etc. represents the deletions of conserved sequences within the Sindbis virus nsP1. The changes to the His(39), Arg(91), Asp(94), or Tyr(249) to Ala resulted in the loss of MTase activity and the ability of the virus to cause infection. Although changes, Ile(369) to Val, to the conserved amino acids did not affect MTase or infection; larger deletions (between 442 and 492) led to a complete loss in the MTase activity. Table 1 represents the results of the mutations on various clones to express MTase activity. | 5 |
| Challenge | Kimberly.heggeset, Team A5 | 2014-04-10 08:34:36 CDT | You need new notes for this annotation | 0 |
| Private Assessment | Suzialeksander | 2014-05-12 14:15:00 CDT | You need to be an instructor to view these notes. | Updated by Instructor |
| Private Assessment | JimHu | 2014-04-20 16:43:23 CDT | You need to be an instructor to view these notes. | Corrected Through Challenges ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
| Public Assessment | DanielRenfro | 2014-04-10 13:50:51 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0008174||mRNA methyltransferase activity||PMID:8610444||IMP: Inferred from Mutant Phenotype||||F||Changes and/or deletions to conserved amino acid sequences in nsP1 resulted in lower Km for S-adenosyl methionine. Reduced methyl donor substrate concentration significantly reduced MTase activity and viral infectivity. Fig. 2 Expression of deleted forms; Table 1 MTase activity expressed; Table 2 infectivity of RNA transcripts||complete | Flagged |
| Public Assessment | DanielRenfro | 2014-04-10 08:34:36 CDT | This annotation has been flagged because it has been edited since last assessment ||GO:0008174||mRNA methyltransferase activity||PMID:8610444||IMP: Inferred from Mutant Phenotype||||F||Changes and/or deletions to conserved amino acid sequences in nsP1 resulted in lower Km for S-adenosyl methionine. Reduced methyl donor substrate concentration significantly reduced MTase activity and viral infectivity. Fig. 2 Expression of deleted forms; Table 1 MTase activity expressed; Table 2 infectivity of RNA transcripts||complete | Flagged |
| Private Assessment | Amcmillan | 2014-03-25 19:38:43 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Qualifier ✔ Go term ✗ Notes ✔ Unique/Original |
