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SINDV:POLN
Contents
| Species (Taxon ID) | Sindbis virus (SINV). (11034) | |
| Gene Name(s) | No Information Provided. | |
| Protein Name(s) | Non-structural polyprotein
Polyprotein nsP1234 P1234 P123 P123' mRNA-capping enzyme nsP1 Non-structural protein 1 Protease nsP2 Non-structural protein 2 nsP2 Non-structural protein 3 nsP3 Non-structural protein 3' nsP3' RNA-directed RNA polymerase nsP4 Non-structural protein 4 nsP4 | |
| External Links | ||
| UniProt | P03317 | |
| EMBL | J02363 | |
| PIR | A03917 | |
| RefSeq | NP_062889.1 | |
| PDB | 4GUA | |
| PDBsum | 4GUA | |
| ProteinModelPortal | P03317 | |
| MEROPS | C09.001 | |
| GeneID | 1502154 | |
| PMAP-CutDB | P03317 | |
| Proteomes | UP000006710 | |
| GO | GO:0044175 GO:0044176 GO:0044188 GO:0042025 GO:0020002 GO:0016020 GO:0005524 GO:0008234 GO:0005525 GO:0004386 GO:0008174 GO:0004651 GO:0003723 GO:0003968 GO:0006370 GO:0039523 GO:0006351 GO:0039694 | |
| Gene3D | 3.40.50.150 | |
| InterPro | IPR027351 IPR002620 IPR002589 IPR027417 IPR007094 IPR029063 IPR002588 IPR001788 | |
| Pfam | PF01661 PF01707 PF00978 PF01443 PF01660 | |
| SMART | SM00506 | |
| SUPFAM | SSF52540 SSF53335 | |
| PROSITE | PS51154 PS51520 PS51657 PS50507 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0006606 |
protein import into nucleus |
ECO:0000314 |
P |
In figure 1A, when the expression of NSP3 was silenced in infected cells, the distribution of mRNAs with a poly(A) tail appeared very similar to that observed in uninfected cells, indicating that NSP3 is involved in the nuclear accumulation both of PABPC and of poly(A)-containing mRNAs. Rubio, R et al. (2013) see PMID reference number |
complete | ||||
| GO:0039690 |
positive stranded viral RNA replication |
ECO:0000315 |
P |
In Fig. 5 |
complete | |||||
| GO:0008174 |
mRNA methyltransferase activity |
ECO:0000315 |
F |
Table 1 represents the results of the mutations on various clones to express MTase activity. Changes and/or deletions to conserved amino acid sequences in nsP1 resulted in lower Km for S-adenosyl methionine. Reduced methyl donor substrate concentration significantly reduced MTase activity and viral infectivity. |
complete | |||||
|
enables |
GO:0008174 |
mRNA methyltransferase activity |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039690 |
positive stranded viral RNA replication |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0001172 |
transcription, RNA-templated |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0003968 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0098507 |
polynucleotide 5' dephosphorylation |
ECO:0000366 |
evidence based on logical inference from automatic annotation used in automatic assertion |
GO:0004651 |
P |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006351 |
transcription, DNA-templated |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006396 |
RNA processing |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008174 |
mRNA methyltransferase activity |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039694 |
viral RNA genome replication |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0080009 |
mRNA methylation |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004651 |
polynucleotide 5'-phosphatase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0017111 |
nucleoside-triphosphatase activity |
ECO:0000501 |
evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016740 |
transferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008168 |
methyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006370 |
7-methylguanosine mRNA capping |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0020002 |
host cell plasma membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0042025 |
host cell nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0016032 |
viral process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044174 |
host cell endosome |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039657 |
suppression by virus of host gene expression |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003968 |
RNA-directed 5'-3' RNA polymerase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016779 |
nucleotidyltransferase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0032259 |
methylation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0008152 |
metabolic process |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044187 |
host cell lysosome |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008233 |
peptidase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0008234 |
cysteine-type peptidase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005525 |
GTP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0004386 |
helicase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0033644 |
host cell membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006397 |
mRNA processing |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006508 |
proteolysis |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003824 |
catalytic activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0039523 |
suppression by virus of host RNA polymerase II activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044157 |
host cell projection |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030430 |
host cell cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044188 |
host cell lysosomal membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044175 |
host cell endosome membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044176 |
host cell filopodium |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Rubio, RM et al. (2013) Rotavirus prevents the expression of host responses by blocking the nucleocytoplasmic transport of polyadenylated mRNAs. J. Virol. 87 6336-45 PubMed GONUTS page
- ↑ 2.0 2.1 Park, E & Griffin, DE (2009) The nsP3 macro domain is important for Sindbis virus replication in neurons and neurovirulence in mice. Virology 388 305-14 PubMed GONUTS page
- ↑ 3.0 3.1 Wang, HL et al. (1996) Mutagenesis of the Sindbis virus nsP1 protein: effects on methyltransferase activity and viral infectivity. Virology 217 527-31 PubMed GONUTS page
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- GO:0004386 ! helicase activity
- GO:0030430 ! host cell cytoplasm
- GO:0044175 ! host cell endosome membrane
- GO:0044174 ! host cell endosome
- GO:0044176 ! host cell filopodium
- GO:0044188 ! host cell lysosomal membrane
- GO:0044187 ! host cell lysosome
- GO:0033644 ! host cell membrane
- GO:0042025 ! host cell nucleus
- GO:0020002 ! host cell plasma membrane
- GO:0044157 ! host cell projection
- GO:0016787 ! hydrolase activity
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