GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
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Cacao
| GO:0006810 | transport | PMID:16923893 | IDA: Inferred from Direct Assay | P | ||||
| This annotation made on page: RICPR:TLCD By: An553775 (group Team Golden) on 2012-02-22 16:30:29 CST. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Ch009031, Team James Watson | 2012-03-25 17:46:29 CDT | The reason for this challenge is because the notes are not explained thoroughly enough. The explanation should be that the Tlc4 gene was cloned into the pET-11a expression vector and introduced into the C41 strain of E. coli by electroporation. Using filtration uptake assays, it was shown, as seen in figure 2A, that Tlc4 was able to transport CTP, UTP, and GDP with very similar maximum rates in the E. coli expression vector. CTP (.1 mM) bound the most avidly followed by UTP (.3 mM) and then GDP (.6 mM). | 0 |
| Private Assessment | Oherrera | 2012-03-04 11:37:00 CST | You need to be an instructor to view these notes. | Requires Changes |
