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Cacao
| GO:0008940 | nitrate reductase activity | PMID:1922075 | IDA: Inferred from Direct Assay | F | ||||
| This annotation made on page: EMENI:NIRA By: Jolive15 (group Team Denitrifiers) on 2011-10-22 22:02:13 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | Lrodgers, Team KremlinOnTheCrum | 2011-10-29 09:37:56 CDT | Figure 5 shows that nirA transcription is constitutive. It does not show that it is involved in nitrate reductase activity. Instead what the paper shows is that nirA is acting as a transcription factor for niaD and niiA. This in turn regulates the steady state concentration of nitrate and nitrite reductase mRNA concentration. | 6 |
| Private Assessment | Mkline | 2012-03-28 12:53:45 CDT | You need to be an instructor to view these notes. | Acceptable |
| Public Assessment | Bmcintosh | 2011-11-18 16:56:02 CST | We agree with the challenge - the experiment does not characterize the activity of the protein, only the mRNA message by Northern blotting. The changes in expression are assessed in various NirA mutants. | Requires Changes |
