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Cacao
| GO:0001216 | DNA-binding transcription activator activity | PMID:17892462 | ECO:0001204 IDA: Inferred from Direct Assay | Molecular Function | Figure 5 shows the results of an In vitro transcription assay in which 20 ng of linearized pGS1886 DNA was incubated with RNA polymerase (RNAP) and differing amounts of H-NS and SlyA. H-NS is shown to inhibit transcription of hlyE in Figure 5C through use of a PCR-amplified DNA fragment extending from −474 to +222 that includes the hlyE promoter, seemingly completely inhibiting it at H-NS concentration of 4.0 μM. Figure 5D shows that transcription can be restored by increasing concentrations of SlyA through using the same In vitro transcription assay process and 2.0 μM of H-NS. At about 1.0 μM of SlyA, hlyE appears to be transcribed at similar levels to the control, containing no H-NS. From this it can be concluded SlyA can compete with H-NS for binding sites upstream and downstream of the hlyE transcriptional start site and restore transcription of hlyE. | complete | ||
| This annotation made on page: ECOLI:SLYA By: MGuldan (group Team Green A 2019) on 2019-03-29 18:28:41 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Public Assessment | Ivanerill | 2019-04-14 13:19:47 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-04-10 12:00:19 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-04-08 17:12:28 CDT | You conclude more than the authors based on Fig. 5. SlyA is shown to bind DNA in other figures, but not here. This is not demonstrating a molecular function. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
