GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| Contributes to | GO:0009253 | peptidoglycan catabolic process | PMID:18348984 | IDA: Inferred from Direct Assay | P | |||
| This annotation made on page: BPT5:TMP By: HPaudel (group Team Green B 2018) on 2018-04-01 17:35:18 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||
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| Challenge | ORichter, Team Purple B 2018 | 2018-04-03 11:55:25 CDT | The description of the evidence is not clearly specified, in fact, HPLC is never mentioned as the method of detection. My correction is below: Beta-galactosidase released through ONPG hydrolysis in Pb2-Cterm-treated E.coli bacteria versus sonicated cells is measured in Figure 6. Figure 6 shows that ONPG hydrolysis proceeds fastest with the sonicated cells compared to the E. coli. It took 50 minutes for the E. coli treated with Pb2-Cterm to reach 60% of the beta-galactosidase released from sonicated bacteria. DDM did not induce the expression of beta-galactosidase upon its addition. Triggering beta-galactosidase expression, therefore, had to be due to a type of peptidoglycan hydrolase activity that was induced by the Pb2-Cterm, which would span both the membrane and the peptidoglycan. The Pb2-Cterm incubated peptidoglycan (Figure 7.a) showed significantly more expression on the HPLC compared to the non-Pb2-Cterm-incubated peptidoglycan HPLC (Figure 7.b). The peptidoglycan hydrolase activity is a peptidoglycan catabolic process so this is accurate. | 1 | ||||||||||||||||||
| Challenge | HPatel, Team Blue B 2018 | 2018-04-03 08:26:29 CDT | I would elaborate your note a little bit more in terms of proving peptidoglycan degradation through the HPLC. The profile was different when Pb2-Cterm was first added and then the mutanolysin. Also, mentioning the 3 major species from the peptidoglycan digestion would make the argument stronger. | 1 | ||||||||||||||||||
| Challenge | TBurnett, Team Blue A 2018 | 2018-04-02 17:04:18 CDT | Peptidoglycan catabolic process is a biological process. It would be more fitting to find a GO Term for molecular function pertaining to the breakdown of peptidoglycan. It may also be useful to state that this was validated through HPLC. | 1 | ||||||||||||||||||
| Public Assessment | Ivanerill | 2018-05-02 09:18:47 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||
| Public Assessment | DanielRenfro | 2018-04-30 09:45:49 CDT | This annotation has been flagged because it has been edited since last assessment
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| Public Assessment | Ivanerill | 2018-04-30 09:44:34 CDT | The “contributes to” qualifier is used to capture the molecular function of complexes when the activity is distributed over several subunits. I don't see evidence of this here. | Requires Changes ✔ Protein ✔ Publication ✗ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes | ||||||||||||||||||
| Private Assessment | Ivanerill | 2018-04-30 06:08:51 CDT | You need to be an instructor to view these notes. | Requires Changes ✔ Protein ✔ Publication ✗ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||
| Public Assessment | DanielRenfro | 2018-04-18 10:18:18 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||
| Public Assessment | DanielRenfro | 2018-04-15 19:31:56 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||
| Public Assessment | Ivanerill | 2018-04-14 20:10:57 CDT | Comments can be more specific on the methodology used and the overall description of the experiment. This is showing evidence of molecular function, not a biological process (since it is in vitro). You can find a term a molecular function term that appropriately reflects this (even though probably not as specific). | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
