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Cacao

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GO:0017053transcriptional repressor complexPMID:12426390IMP: Inferred from Mutant Phenotype C
This annotation made on page: BPPH2:NP
By: Puglisk1 (group Team Blue-B) on 2017-04-05 10:13:51 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeGarlingrk,
Team ZIRTS
2017-04-12 09:12:30 CDT

Figure 1 is a gel shift assay showing complex formation in mutant and wild-type phenotype. The notes claim formation of the repression complex stops transcription in the Bacillus subtilis phage phi 29, but that is not what the figure is indicating.

2
ChallengeSyedsa,
Team Viridae
2017-04-10 12:06:43 CDT

To strengthen this annotation, more specificity is needed to know how the two mutations effect p4 and p6. More information is needed from figure 1a on the locations and positions of the promoter regions. Expand on the gel bands and the difference of the mass on the repressor complex seen in figure 1b.

0
Private
Assessment
Ivanerill2017-05-25 04:53:56 CDTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
Ivanerill2017-05-24 06:10:34 CDT

Comments have been updated, but WITH field is still missing

Requires Changes
With/From
Notes
Public
Assessment
DanielRenfro2017-04-17 10:00:04 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0017053 transcriptional repressor complex PMID:12426390 IMP: Inferred from Mutant Phenotype C Figure 1 displays the mutations of protein p4 and their effects on forming the repression complex between proteins p4 and p6 that represses early transcription in Bacillus subtilis phage phi 29. These mutations disallow the formation of a stable complex, hence the repressor complex cannot be formed between proteins p4 and p6 to completely halt early transcription and switch to late transcription. complete
CACAO 12437
on BPPH2:NP
Flagged
Public
Assessment
Ivanerill2017-04-15 17:12:13 CDT

GO:0017053 should be defined with the identifier of the other protein forming complex in the WITH field.

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original