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Cacao

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Contributes toGO:0003678DNA helicase activityPMID:18157148IDA: Inferred from Direct Assay F
This annotation made on page: BPSPP:Q38152
By: Flounlackerkm (group Team DirtyChubi) on 2016-04-11 11:32:59 CDT.



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Team DirtyChubi0




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment
Suzialeksander2016-06-27 16:12:24 CDTYou need to be an instructor to view these notes.Updated by Instructor
Private
Assessment
Suzialeksander2016-05-23 13:56:38 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Public
Assessment
AAJohnson2016-05-09 08:44:30 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-05-06 12:17:45 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
Contributes to GO:0003678 DNA helicase activity PMID:18157148 IDA: Inferred from Direct Assay F Fig. 1 shows the hexameric structure of the DNA helicase protein for bacillus phage SPP1 determined by using x-ray crystallography. Fig. 2 shows the ATP binding domain that provides the protein with the energy needed to unwind the DNA, this feature is also shown in fig. 3d. This information was supported by supplementary fig 1, where the structure was crystallized in non-hydrolyzable ATP to show the ATP binding residues. complete
CACAO 11623
on BPSPP:Q38152
Flagged
Public
Assessment
AAJohnson2016-05-03 15:43:32 CDT

My thought as I read your notes is "but, how do I know that is helicase and how does fig 1 show helicase activity (your chosen GO term)?" Your notes should be improved by the addition of supporting figures such as figure 3d that shows a flexible loop involved in DNA unwinding, and supplementary figure 1 where the protein was crystallized with a nonhydrolyzable ATP mimic to show ATP binding residues. Please examine these figures and use your own words to describe how they support your annotation.

Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original