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Cacao

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GO:0031167rRNA methylationPMID:9527769IMP: Inferred from Mutant Phenotype P
This annotation made on page: STRPY:O07480
By: Kaitlynn.Jasken (group Team I'm bringing sexy bacterium) on 2016-03-04 18:25:08 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeKaitlynn.Jasken,
Team I'm bringing sexy bacterium		2016-04-04 18:13:42 CDT

Based on my notes and figure it tells me that the evidence code that should be used is ISA because the ermTR gene is aligned with the ermA gene with the nucleotide sequence so that means I should have my with/from with the uniport ID: Q2XU33. This ID is for the ermA gene that the ermTR gene is aligned with. I could have used the ISO if I focused on the ermAM gene which is brought up in the PCR sequencing experiments. But in this annotation I am focusing on the ermTR gene.

1
ChallengeNatjack1,
Team Green B		2016-04-04 17:24:34 CDT

The reference describes sequence orthology between Streptococcus pyogenes and Streptococcus pneumoniae. But because the data is a percent comparison to another organisms known function, the annotation requires a with/from field referring to the known gene function.

1
Private
Assessment		Suzialeksander2016-06-13 14:25:52 CDTYou need to be an instructor to view these notes.Unacceptable
Public
Assessment		Suzialeksander2016-04-06 15:13:45 CDT

So, when I look up your "match" protein (http://www.ebi.ac.uk/QuickGO/GProtein?ac=Q2XU33), there aren't any manual annotations, only IEA (electronic ones). You need your "match" protein (Q2XU33) to have an annotation to GO:0031167 rRNA methylation in order for this one to be accepted. The good news is if you make this, then they will both count!

Requires Changes
Public
Assessment		DanielRenfro2016-04-04 18:13:42 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment		DanielRenfro2016-04-04 17:24:34 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment		Suzialeksander2016-03-29 15:17:43 CDTYou need to be an instructor to view these notes.Requires Changes
Public
Assessment		DanielRenfro2016-03-28 21:24:42 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISA: Inferred from Sequence Alignment UniProtKB:Q2XU33 P Figure 1 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. complete
CACAO 11349
on STRPY:O07480
Flagged
Public
Assessment		DanielRenfro2016-03-28 21:22:26 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISA: Inferred from Sequence Alignment UniProtKB:Q8GPL2 P Figure 1 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. complete
CACAO 11349
on STRPY:O07480
Flagged
Private
Assessment		Suzialeksander2016-03-28 14:35:58 CDTYou need to be an instructor to view these notes.Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✗ Evidence
✗ With/From
Public
Assessment		DanielRenfro2016-03-23 11:28:01 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISO: Inferred from Sequence Orthology UniProtKB:Q8GPL2 P Figure 1 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. complete
CACAO 11349
on STRPY:O07480
Flagged
Public
Assessment		DanielRenfro2016-03-23 11:17:57 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISO: Inferred from Sequence Orthology UniProtKB:A0A067YRU6 P Figure 1 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. complete
CACAO 11349
on STRPY:O07480
Flagged
Public
Assessment		DanielRenfro2016-03-23 10:14:05 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISO: Inferred from Sequence Orthology UniProtKB:A0A067YRU6 P Figure 2 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. complete
CACAO 11349
on STRPY:O07480
Flagged
Public
Assessment		DanielRenfro2016-03-23 10:11:01 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISO: Inferred from Sequence Orthology P Figure 2 shows that the nucleotide sequence of the ermTR gene in S. pyogenes. It is aligned with the ermA gene. Within the Epidemiology of the ermTR gene section states that, "These studies suggest that ermTR may be widely distributed among streptococci with MLSb resistance. Missing: with/from
CACAO 11349
on STRPY:O07480
Flagged
Public
Assessment		DanielRenfro2016-03-23 09:47:52 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031167 rRNA methylation PMID:9527769 ISO: Inferred from Sequence Orthology P Table 1 shows you the genes that are within the S. pyogenes. While table 2 shows you the Oligonucleotide primers used in PCR and sequencing. There is also a nucleotide sequence accession number for the ermTR gene at the bottom of the materials and methods which if clicked on shows that this gene is Erythromycin Resistant. Missing: with/from
CACAO 11349
on STRPY:O07480
Flagged
Private
Assessment		Min.lorna2016-03-08 19:34:04 CSTYou need to be an instructor to view these notes.Flagged
Private
Assessment		Min.lorna2016-03-07 17:46:31 CSTYou need to be an instructor to view these notes.Flagged
Private
Assessment		Min.lorna2016-03-06 14:49:21 CSTYou need to be an instructor to view these notes.Flagged


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