SHIFL:IPA4

Species (Taxon ID)	Shigella flexneri. (623)
Gene Name(s)	ipaH4.5
Protein Name(s)	Probable E3 ubiquitin-protein ligase ipaH4.5 Probable RING-type E3 ubiquitin transferase ipaH4.5 (ECO:0000305)
External Links
UniProt	P18009
EMBL	M76445 AL391753 AF348706 AF386526 M32063
PIR	S18248
RefSeq	NP_085239.1 NP_858212.1 WP_010921638.1 YP_009062470.1
ProteinModelPortal	P18009
PaxDb	P18009
PRIDE	P18009
EnsemblBacteria	AAL72347
GeneID	1238050 876707
KEGG	sfl:CP0079
PATRIC	fig|198214.7.peg.5326
eggNOG	ENOG4108QHK COG4886
HOGENOM	HOG000290475
OMA	DSCEDRI
Proteomes	UP000001006
GO	GO:0005576 GO:0030430 GO:0004842 GO:0009405
Gene3D	3.80.10.10
InterPro	IPR032675 IPR001611 IPR032674 IPR029487
Pfam	PF14496 PF12468
SUPFAM	SSF52058
PROSITE	PS51450

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0044074	negative regulation by symbiont of host translation	PMID:23083102	ECO:0000314			P		organism: Shigella flexneri Fig 5A shows that cells with IPA4 had less transcriptional activity of NF-κB. "IpaH4.5 inhibits the transcriptional activity of NF-κB. "	complete CACAO 12965
	GO:0052170	negative regulation by symbiont of host innate immune response	PMID:26700764	ECO:0000315			P		Shigella flexneri Figure 1A and 1B show that the IPA4 lowers IRF3 activity which is a vital component of host immune response. The delta IPA4, inversely, shows an increase in IRF3 activity in figure 1B which is expected if IPA4 negatively regulates host immune response	complete CACAO 12966
	GO:0006511	ubiquitin-dependent protein catabolic process	PMID:26700764	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Shigella flexneri Protein is IPA4 but referred to as IPAH4.5 in paper. Figure 5A-E: "The results showed that TBK1 was ubiquitylated in vitro by IpaH4.5" and Figure 5A-E show that "paH4.5 induces TBK1 degradation by K48-linked ubiquitination"	complete CACAO 12967
	GO:0051865	protein autoubiquitination	PMID:23083102	ECO:0000314			P		Shigella flexneri The author concludes from figure 4A that IPA4 can autoubiquitinate from a western blot "Thus, IpaH4.5 exhibited the ability to (i) autoubiquitinate and (ii) polyubiquitinate HA-tagged ubiquitin (Fig. 4A... "	complete CACAO 12983
	GO:0000209	protein polyubiquitination	PMID:23083102	ECO:0000314			P		Shigella flexneri Figure 4B is a western blot that shows that IPA4 is able to polyubiquitinate " Thus, IpaH4.5 exhibited the ability to (i) autoubiquitinate and (ii) polyubiquitinate HA-tagged ubiquitin (Fig. 4A and B)."	complete CACAO 12984
part_of	GO:0005576	extracellular region	PMID:11418613	ECO:0000314	direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
	GO:0052083	negative regulation by symbiont of host cell-mediated immune response	PMID:23083102	ECO:0000315			P		Organism: Shigella flexneri protein: IPA4 but called IPAH4.5 in paper. Fig 2C: Wild type IPA4 shows a lower cytokine production in the host when compared to delta IPA4 and the mutated IPA4 thus showing negative regulation of cytokine secretion by the symbiont (s. flexneri). The modulation of cytokine production is proved by the mutant, which has a higher cytokine production than the wild type IPA4. Cytokine production is part of the cell mediated immune response in the host meaning that IPA4 is negatively regulating the response.	complete CACAO 13018
enables	GO:0004842	ubiquitin-protein transferase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR029487	F		Seeded From UniProt	complete
involved_in	GO:0016567	protein ubiquitination	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR029487	P		Seeded From UniProt	complete
part_of	GO:0030430	host cell cytoplasm	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-1035 UniProtKB-SubCell:SL-0381	C		Seeded From UniProt	complete
enables	GO:0016740	transferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0808	F		Seeded From UniProt	complete
part_of	GO:0005576	extracellular region	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0964 UniProtKB-SubCell:SL-0243	C		Seeded From UniProt	complete
involved_in	GO:0009405	pathogenesis	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0843	P		Seeded From UniProt	complete
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References

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