SCHPO:SAP1

Species (Taxon ID)	Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812)
Gene Name(s)	sap1
Protein Name(s)	Switch-activating protein 1
External Links
UniProt	P40847
EMBL	X77578 CU329672
PIR	A56214 T45515
RefSeq	NP_587873.1
PDB	5B7J 5JDK 6EXT 6EXU
PDBsum	5B7J 5JDK 6EXT 6EXU
ProteinModelPortal	P40847
SMR	P40847
BioGrid	275853
STRING	4896.SPCC1672.02c.1
iPTMnet	P40847
MaxQB	P40847
PaxDb	P40847
PRIDE	P40847
EnsemblFungi	SPCC1672.02c.1
GeneID	2539285
KEGG	spo:SPCC1672.02c
EuPathDB	FungiDB:SPCC1672.02c
PomBase	SPCC1672.02c
OMA	IHTESAC
PRO	PR:P40847
Proteomes	UP000002485
GO	GO:0000790 GO:0005634 GO:0003677 GO:0008301 GO:0043110 GO:0043565 GO:1990837 GO:0007059 GO:0000728 GO:0031582 GO:0071170

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0042803	protein homodimerization activity	PMID:16166653^[1]	ECO:0000304			F			complete
	GO:0043110	rDNA spacer replication fork barrier binding	PMID:16166653^[1]	ECO:0000314			F			complete
	GO:0051276	chromosome organization	PMID:14555473^[2]	ECO:0000315			P			complete
	GO:0000790	nuclear chromatin	PMID:14555473^[2]	ECO:0000304			C			complete
	GO:0008301	DNA bending activity	PMID:16195226^[3]	ECO:0000314			F			complete
	GO:0043110	rDNA spacer replication fork barrier binding	PMID:16195226^[3]	ECO:0000314			F			complete
	GO:0007126	meiosis	PMID:17072883^[4]	ECO:0000303			P			complete
	GO:0071170	site-specific DNA replication termination	PMID:16166653^[1]	ECO:0000314			P			complete
	GO:0000728	gene conversion at mating-type locus, DNA double-strand break formation	PMID:1915277^[5]	ECO:0000315			P			complete
	GO:0003677	DNA binding	PMID:16166653^[1]	ECO:0000314			F			complete
	GO:0031582	replication fork arrest at rDNA repeats	PMID:16166653^[1]	ECO:0000314			P			complete
	GO:0003677	DNA binding	PMID:8065904^[6]	ECO:0000314			F			complete
	GO:0031582	replication fork arrest at rDNA repeats	PMID:16195226^[3]	ECO:0000315			P			complete
	GO:0008156	negative regulation of DNA replication	PMID:21151105^[7]	ECO:0000315			P		Fig. 1	complete
	GO:0005634	nucleus	PMID:16823372^[8]	ECO:0000314			C			complete
	GO:0000728	gene conversion at mating-type locus, DNA double-strand break formation	PMID:1915277^[5]	ECO:0000315			P			complete
	GO:1902985	mitotic pre-replicative complex assembly	PMID:28223353^[9]	ECO:0000315			P		Figure 5. Sap1 is required to load Cdc18 onto DNA for pre-RC assembly. Sap1, S. pombe.	complete CACAO 12699
	GO:0000790	nuclear chromatin	PMID:14555473^[2]	ECO:0000304			C			complete
enables	GO:1990837	sequence-specific double-stranded DNA binding	PMID:29610759^[10]	ECO:0000314	direct assay evidence used in manual assertion		F	occurs_at:(SO:0001789) occurs_in:(CL:0002674)	Seeded From UniProt	complete
involved_in	GO:0071170	site-specific DNA replication termination	PMID:16166653^[1]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:8114737^[11]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
	GO:0003677	DNA binding	PMID:16166653^[1]	ECO:0000314			F			complete
	GO:0003677	DNA binding	PMID:8065904^[6]	ECO:0000314			F			complete
enables	GO:0043565	sequence-specific DNA binding	PMID:8065904^[6]	ECO:0000314	direct assay evidence used in manual assertion		F	occurs_at:(SO:0001864)	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:7651412^[12]	ECO:0000314	direct assay evidence used in manual assertion		F	occurs_at:(SO:0001864)	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:1915277^[5]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
	GO:0005634	nucleus	PMID:16823372^[8]	ECO:0000314			C			complete
enables	GO:0043110	rDNA spacer replication fork barrier binding	PMID:16195226^[3]	ECO:0000314	direct assay evidence used in manual assertion		F	occurs_at:(SO:0001864)	Seeded From UniProt	complete
involved_in	GO:0031582	replication fork arrest at rDNA repeats	PMID:16195226^[3]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
	GO:0008301	DNA bending activity	PMID:16195226^[3]	ECO:0000314			F			complete
involved_in	GO:0031582	replication fork arrest at rDNA repeats	PMID:16166653^[1]	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
	GO:0031582	replication fork arrest at rDNA repeats	PMID:16166653^[1]	ECO:0000314			P			complete
enables	GO:0008301	DNA binding, bending	PMID:16195226^[3]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
	GO:0031582	replication fork arrest at rDNA repeats	PMID:16195226^[3]	ECO:0000315			P			complete
part_of	GO:0005634	nucleus	PMID:16823372^[8]	ECO:0007005	high throughput direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
	GO:0042803	protein homodimerization activity	PMID:16166653^[1]	ECO:0000304			F			complete
enables	GO:0003677	DNA binding	PMID:16166653^[1]	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
	GO:0043110	rDNA spacer replication fork barrier binding	PMID:16166653^[1]	ECO:0000314			F			complete
part_of	GO:0000790	nuclear chromatin	PMID:29610759^[10]	ECO:0000314	direct assay evidence used in manual assertion		C	coincident_with:(SO:0002158) part_of:(CL:0002674)	Seeded From UniProt	complete
	GO:0043110	rDNA spacer replication fork barrier binding	PMID:16195226^[3]	ECO:0000314			F			complete
part_of	GO:0000790	nuclear chromatin	PMID:29610759^[10]	ECO:0000314	direct assay evidence used in manual assertion		C	coincident_with:(SO:0001789) part_of:(CL:0002674)	Seeded From UniProt	complete
involved_in	GO:0000728	gene conversion at mating-type locus, DNA double-strand break formation	PMID:1915277^[5]	ECO:0000315	mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0007059	chromosome segregation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0159	P		Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F		Seeded From UniProt	complete
part_of	GO:0005634	nucleus	GO_REF:0000037 GO_REF:0000039	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0539 UniProtKB-SubCell:SL-0191	C		Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Mejía-Ramírez, E et al. (2005) The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast. Mol. Cell. Biol. 25 8755-61 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 de Lahondès, R et al. (2003) Fission yeast Sap1 protein is essential for chromosome stability. Eukaryotic Cell 2 910-21 PubMed GONUTS page
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 Krings, G & Bastia, D (2005) Sap1p binds to Ter1 at the ribosomal DNA of Schizosaccharomyces pombe and causes polar replication fork arrest. J. Biol. Chem. 280 39135-42 PubMed GONUTS page
↑ Aslett, M & Wood, V (2006) Gene Ontology annotation status of the fission yeast genome: preliminary coverage approaches 100%. Yeast 23 913-9 PubMed GONUTS page
↑ ^5.0 ^5.1 ^5.2 ^5.3 Arcangioli, B & Klar, AJ (1991) A novel switch-activating site (SAS1) and its cognate binding factor (SAP1) required for efficient mat1 switching in Schizosaccharomyces pombe. EMBO J. 10 3025-32 PubMed GONUTS page
↑ ^6.0 ^6.1 ^6.2 Arcangioli, B et al. (1994) Identification of the DNA-binding domains of the switch-activating-protein Sap1 from S.pombe by random point mutations screening in E.coli. Nucleic Acids Res. 22 2930-7 PubMed GONUTS page
↑ Zaratiegui, M et al. (2011) CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR. Nature 469 112-5 PubMed GONUTS page
↑ ^8.0 ^8.1 ^8.2 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
↑ Guan, L et al. (2017) Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 292 6056-6075 PubMed GONUTS page
↑ ^10.0 ^10.1 ^10.2 Raimondi, C et al. (2018) Molecular signature of the imprintosome complex at the mating-type locus in fission yeast. Microb Cell 5 169-183 PubMed GONUTS page
↑ Arcangioli, B et al. (1994) Sap1, a protein that binds to sequences required for mating-type switching, is essential for viability in Schizosaccharomyces pombe. Mol. Cell. Biol. 14 2058-65 PubMed GONUTS page
↑ Ghazvini, M et al. (1995) The essential DNA-binding protein sap1 of Schizosaccharomyces pombe contains two independent oligomerization interfaces that dictate the relative orientation of the DNA-binding domain. Mol. Cell. Biol. 15 4939-46 PubMed GONUTS page

[PMID:16166653-1] 1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Mejía-Ramírez, E et al. (2005) The mating type switch-activating protein Sap1 Is required for replication fork arrest at the rRNA genes of fission yeast. Mol. Cell. Biol. 25 8755-61 PubMed GONUTS page

[PMID:14555473-2] 2.0 ^2.1 ^2.2 de Lahondès, R et al. (2003) Fission yeast Sap1 protein is essential for chromosome stability. Eukaryotic Cell 2 910-21 PubMed GONUTS page

[PMID:16195226-3] 3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 Krings, G & Bastia, D (2005) Sap1p binds to Ter1 at the ribosomal DNA of Schizosaccharomyces pombe and causes polar replication fork arrest. J. Biol. Chem. 280 39135-42 PubMed GONUTS page

[PMID:17072883-4] Aslett, M & Wood, V (2006) Gene Ontology annotation status of the fission yeast genome: preliminary coverage approaches 100%. Yeast 23 913-9 PubMed GONUTS page

[PMID:1915277-5] 5.0 ^5.1 ^5.2 ^5.3 Arcangioli, B & Klar, AJ (1991) A novel switch-activating site (SAS1) and its cognate binding factor (SAP1) required for efficient mat1 switching in Schizosaccharomyces pombe. EMBO J. 10 3025-32 PubMed GONUTS page

[PMID:8065904-6] 6.0 ^6.1 ^6.2 Arcangioli, B et al. (1994) Identification of the DNA-binding domains of the switch-activating-protein Sap1 from S.pombe by random point mutations screening in E.coli. Nucleic Acids Res. 22 2930-7 PubMed GONUTS page

[PMID:21151105-7] Zaratiegui, M et al. (2011) CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR. Nature 469 112-5 PubMed GONUTS page

[PMID:16823372-8] 8.0 ^8.1 ^8.2 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page

[PMID:28223353-9] Guan, L et al. (2017) Sap1 is a replication-initiation factor essential for the assembly of pre-replicative complex in the fission yeast Schizosaccharomyces pombe. J. Biol. Chem. 292 6056-6075 PubMed GONUTS page

[PMID:29610759-10] 10.0 ^10.1 ^10.2 Raimondi, C et al. (2018) Molecular signature of the imprintosome complex at the mating-type locus in fission yeast. Microb Cell 5 169-183 PubMed GONUTS page

[PMID:8114737-11] Arcangioli, B et al. (1994) Sap1, a protein that binds to sequences required for mating-type switching, is essential for viability in Schizosaccharomyces pombe. Mol. Cell. Biol. 14 2058-65 PubMed GONUTS page

[PMID:7651412-12] Ghazvini, M et al. (1995) The essential DNA-binding protein sap1 of Schizosaccharomyces pombe contains two independent oligomerization interfaces that dictate the relative orientation of the DNA-binding domain. Mol. Cell. Biol. 15 4939-46 PubMed GONUTS page

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SCHPO:SAP1

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