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SCHPO:LEM2
Contents
| Species (Taxon ID) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812) | |
| Gene Name(s) | lem2 (synonyms: heh1) | |
| Protein Name(s) | Lap-Emerin-Man domain protein 2
LEM domain protein 2 | |
| External Links | ||
| UniProt | Q10109 | |
| EMBL | CU329670 | |
| PIR | T37923 | |
| RefSeq | NP_593673.1 | |
| PDB | 5YCA | |
| PDBsum | 5YCA | |
| ProteinModelPortal | Q10109 | |
| SMR | Q10109 | |
| BioGrid | 279001 | |
| STRING | 4896.SPAC18G6.10.1 | |
| iPTMnet | Q10109 | |
| MaxQB | Q10109 | |
| PaxDb | Q10109 | |
| PRIDE | Q10109 | |
| EnsemblFungi | SPAC18G6.10.1 | |
| GeneID | 2542544 | |
| KEGG | spo:SPAC18G6.10 | |
| EuPathDB | FungiDB:SPAC18G6.10 | |
| PomBase | SPAC18G6.10 | |
| InParanoid | Q10109 | |
| OMA | AWYRQEK | |
| PRO | PR:Q10109 | |
| Proteomes | UP000002485 | |
| GO | GO:0061638 GO:0099115 GO:0005639 GO:0044732 GO:1904834 GO:0005635 GO:1902377 GO:0005724 GO:0097038 GO:1990578 GO:0030702 GO:0000183 GO:0099114 GO:0097240 GO:0070197 GO:0006998 GO:0071765 | |
| InterPro | IPR025856 IPR018996 | |
| Pfam | PF12949 PF09402 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0140462 |
pericentric heterochromatin organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Figure 1C Quantitative analysis by RT-qPCR revealed that lem2Δ cells display a twofold increase in reporter gene expression needs relation |
complete | ||||
|
NOT |
GO:0010629 |
negative regulation of gene expression |
ECO:0000315 |
P |
Lem2 negative regulation of gene expression |
Missing: reference | ||||
|
NOT |
GO:0010629 |
negative regulation of gene expression |
ECO:0000315 |
P |
Lem2 is deleted and there is more transcription |
complete | ||||
| GO:0097355 |
protein localization to heterochromatin |
ECO:0000315 |
P |
Figure 5A and 5B: S. pombe with loss of heh1 (Lem2; Lap-Emerin-Man domain protein 2) or nur1 show decreased levels of H3K9me2 and Swi6 at the centromere. |
complete | |||||
| GO:0034506 |
chromosome, centromeric core domain |
ECO:0000314 |
C |
Organism: S. pombe Protein: Lem2//Lap-Emerin-Man domain protein 2 Due to the interactions with the centromeres in Figure 2, the Lem2 protein is shown to be in the nucleus. |
complete | |||||
|
part_of |
GO:1990578 |
perinuclear endoplasmic reticulum membrane |
ECO:0007005 |
high throughput direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:1990141 |
chromatin silencing at centromere outer repeat region |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
occurs_at:(SO:0001799) |
Seeded From UniProt |
complete | ||
|
part_of |
GO:1904834 |
nuclear CENP-A containing chromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:1902377 |
nuclear rDNA heterochromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0099114 |
chromatin silencing at subtelomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0099114 |
chromatin silencing at subtelomere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0097240 |
chromosome attachment to the nuclear envelope |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0097038 |
perinuclear endoplasmic reticulum |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0072766 |
centromere clustering at the mitotic nuclear envelope |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
NOT|involved_in |
GO:0072766 |
centromere clustering at the mitotic nuclear envelope |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0071765 |
nuclear inner membrane organization |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
PomBase:SPCC737.03c |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0070197 |
meiotic attachment of telomere to nuclear envelope |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0061638 |
CENP-A containing chromatin |
ECO:0000269 |
experimental evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0044732 |
mitotic spindle pole body |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
exists_during:(GO:0000092) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0034398 |
telomere tethering at nuclear periphery |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0031618 |
nuclear pericentric heterochromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
coincident_with:(SO:0001796) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0030702 |
chromatin silencing at centromere |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0030466 |
chromatin silencing at silent mating-type cassette |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0006998 |
nuclear envelope organization |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005724 |
nuclear telomeric heterochromatin |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005639 |
integral component of nuclear inner membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005639 |
integral component of nuclear inner membrane |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005635 |
nuclear envelope |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005635 |
nuclear envelope |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003682 |
chromatin binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
occurs_at:(SO:0001796) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000183 |
chromatin silencing at rDNA |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0034507 |
chromosome, centromeric outer repeat region |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:1990141 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0099115 |
chromosome, subtelomeric region |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0099114 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0099115 |
chromosome, subtelomeric region |
ECO:0000364 |
evidence based on logical inference from manual annotation used in automatic assertion |
GO:0099114 |
C |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0016021 |
integral component of membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0051321 |
meiotic cell cycle |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016020 |
membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005637 |
nuclear inner membrane |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Barrales, RR et al. (2016) Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2. Genes Dev. 30 133-48 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Banday, S et al. (2016) Role of Inner Nuclear Membrane Protein Complex Lem2-Nur1 in Heterochromatic Gene Silencing. J. Biol. Chem. 291 20021-9 PubMed GONUTS page
- ↑ Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 Tange, Y et al. (2016) Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions. Genes Cells 21 812-32 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 5.3 Hiraoka, Y et al. (2011) Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres. Genes Cells 16 1000-11 PubMed GONUTS page
- ↑ 6.0 6.1 Gonzalez, Y et al. () Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina. Nucleus 3 60-76 PubMed GONUTS page
- ↑ King, MC et al. (2008) A network of nuclear envelope membrane proteins linking centromeres to microtubules. Cell 134 427-38 PubMed GONUTS page
c
- GO:0061638 ! CENP-A containing chromatin
- GO:0072766 ! centromere clustering at the mitotic interphase nuclear envelope
- GO:0003682 ! chromatin binding
- GO:1990141 ! chromatin silencing at centromere outer repeat region
- GO:0099114 ! chromatin silencing at subtelomere
- GO:0030702 ! chromatin silencing at centromere
- GO:0034506 ! chromosome, centromeric core domain
- GO:0034507 ! chromosome, centromeric outer repeat region
- GO:0099115 ! chromosome, subtelomeric region
- GO:0097240 ! chromosome attachment to the nuclear envelope
d
f
m
n
- GO:0010629 ! negative regulation of gene expression
- GO:1904834 ! nuclear CENP-A containing chromatin
- GO:0006998 ! nuclear envelope organization
- GO:0005635 ! nuclear envelope
- GO:0005637 ! nuclear inner membrane
- GO:0071765 ! nuclear inner membrane organization
- GO:0031618 ! nuclear pericentric heterochromatin
- GO:1902377 ! nuclear rDNA heterochromatin
- GO:0005634 ! nucleus
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