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SCHPO:LEM2

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Species (Taxon ID) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812)
Gene Name(s) lem2 (synonyms: heh1)
Protein Name(s) Lap-Emerin-Man domain protein 2

LEM domain protein 2

External Links
UniProt Q10109
EMBL CU329670
PIR T37923
RefSeq NP_593673.1
ProteinModelPortal Q10109
BioGrid 279001
MINT MINT-4696993
iPTMnet Q10109
MaxQB Q10109
EnsemblFungi [example_ID SPAC18G6.10.1]
GeneID 2542544
KEGG spo:SPAC18G6.10
EuPathDB FungiDB:SPAC18G6.10
PomBase SPAC18G6.10
InParanoid Q10109
OMA NIFREEC
OrthoDB EOG7GBG5Q
NextBio 20803596
PRO PR:Q10109
Proteomes UP000002485
GO GO:0005639
GO:0044732
GO:0005635
GO:0070197
GO:0000070
GO:0006998
GO:0071765
InterPro IPR025856
IPR018996
Pfam PF12949
PF09402

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000070

mitotic sister chromatid segregation

GO_REF:0000024

ISO: Inferred from Sequence Orthology

SGD:S000004497

P

Seeded From UniProt

GO:0003674

molecular_function

GO_REF:0000015

ND: No biological Data available

-

F

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

GO:0005635

nuclear envelope

PMID:16823372[1]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005635

nuclear envelope

PMID:21880100[2]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0005637

nuclear inner membrane

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0179

C

Seeded From UniProt

GO:0005639

integral component of nuclear inner membrane

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018996

C

Seeded From UniProt

GO:0005639

integral component of nuclear inner membrane

PMID:18692466[3]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0006998

nuclear envelope organization

PMID:22540024[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0016020

membrane

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0472

C

Seeded From UniProt

GO:0016021

integral component of membrane

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0812

C

Seeded From UniProt

GO:0044732

mitotic spindle pole body

PMID:21880100[2]

IDA: Inferred from Direct Assay

-

C

Seeded From UniProt

GO:0051321

meiotic cell cycle

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0469

P

Seeded From UniProt

GO:0070197

meiotic attachment of telomere to nuclear envelope

PMID:22540024[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0071765

nuclear inner membrane organization

PMID:21880100[2]

IGI: Inferred from Genetic Interaction

PomBase:SPCC737.03c

P

Seeded From UniProt

GO:0072766

centromere clustering at the nuclear envelope

PMID:21880100[2]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0031937

positive regulation of chromatin silencing

PMID:26744419[5]

IMP: Inferred from Mutant Phenotype

P

Figure 1C Quantitative analysis by RT-qPCR revealed that lem2Δ cells display a twofold increase in reporter gene expression

complete
CACAO 11261

NOT

GO:0010629

negative regulation of gene expression

IMP: Inferred from Mutant Phenotype

P

Lem2 negative regulation of gene expression

Missing: reference
CACAO 11791

NOT

GO:0010629

negative regulation of gene expression

PMID:26744419[5]

IMP: Inferred from Mutant Phenotype

P

Lem2 is deleted and there is more transcription

complete
CACAO 11794

GO:0097355

protein localization to heterochromatin

PMID:27451393[6]

IMP: Inferred from Mutant Phenotype

P

Figure 5A and 5B: S. pombe with loss of heh1 (Lem2; Lap-Emerin-Man domain protein 2) or nur1 show decreased levels of H3K9me2 and Swi6 at the centromere.

complete
CACAO 12389

GO:0034506

chromosome, centromeric core domain

PMID:27451393[6]

IDA: Inferred from Direct Assay

C

Organism: S. pombe Protein: Lem2//Lap-Emerin-Man domain protein 2 Due to the interactions with the centromeres in Figure 2, the Lem2 protein is shown to be in the nucleus.

complete
CACAO 12390

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
  2. 2.0 2.1 2.2 2.3 Hiraoka, Y et al. (2011) Inner nuclear membrane protein Ima1 is dispensable for intranuclear positioning of centromeres. Genes Cells 16 1000-11 PubMed GONUTS page
  3. King, MC et al. (2008) A network of nuclear envelope membrane proteins linking centromeres to microtubules. Cell 134 427-38 PubMed GONUTS page
  4. 4.0 4.1 Gonzalez, Y et al. () Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina. Nucleus 3 60-76 PubMed GONUTS page
  5. 5.0 5.1 Barrales, RR et al. (2016) Control of heterochromatin localization and silencing by the nuclear membrane protein Lem2. Genes Dev. 30 133-48 PubMed GONUTS page
  6. 6.0 6.1 Banday, S et al. (2016) Role of Inner Nuclear Membrane Protein Complex Lem2-Nur1 in Heterochromatic Gene Silencing. J. Biol. Chem. 291 20021-9 PubMed GONUTS page