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PMID:22540024

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Citation

Gonzalez, Y, Saito, A and Sazer, S Fission yeast Lem2 and Man1 perform fundamental functions of the animal cell nuclear lamina. Nucleus 3:60-76

Abstract

In animal cells the nuclear lamina, which consists of lamins and lamin-associated proteins, serves several functions: it provides a structural scaffold for the nuclear envelope and tethers proteins and heterochromatin to the nuclear periphery. In yeast, proteins and large heterochromatic domains including telomeres are also peripherally localized, but there is no evidence that yeast have lamins or a fibrous nuclear envelope scaffold. Nonetheless, we found that the Lem2 and Man1 proteins of the fission yeast Schizosaccharomyces pombe, evolutionarily distant relatives of the Lap2/Emerin/Man1 (LEM) sub-family of animal cell lamin-associated proteins, perform fundamental functions of the animal cell lamina. These integral inner nuclear membrane localized proteins, with nuclear localized DNA binding Helix-Extension-Helix (HEH) domains, impact nuclear envelope structure and integrity, are essential for the enrichment of telomeres at the nuclear periphery and by means of their HEH domains anchor chromatin, most likely transcriptionally repressed heterochromatin, to the nuclear periphery. These data indicate that the core functions of the nuclear lamina are conserved between fungi and animal cells and can be performed in fission yeast, without lamins or other intermediate filament proteins.

Links

PubMed PMC3337167 Online version:10.4161/nucl.18824

Keywords

Animals; Microtubules/metabolism; Nuclear Envelope/metabolism; Nuclear Lamina/metabolism; Protein Transport; Schizosaccharomyces/cytology; Schizosaccharomyces/metabolism; Schizosaccharomyces pombe Proteins/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

SCHPO:MUG61

involved_in

GO:0071763: nuclear membrane organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:MUG61

enables

GO:0062239: heterochromatin-nuclear membrane anchor activity

ECO:0000269: experimental evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:LEM2

involved_in

GO:0071763: nuclear membrane organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:LEM2

enables

GO:0062239: heterochromatin-nuclear membrane anchor activity

ECO:0000269: experimental evidence used in manual assertion

F

Seeded From UniProt

complete

SCHPO:LEM2

involved_in

GO:0006998: nuclear envelope organization

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

SCHPO:LEM2

involved_in

GO:0070197: meiotic attachment of telomere to nuclear envelope

ECO:0000315: mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

Notes

See also

References

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