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PMID:9930758
| Citation |
Takeuchi, Y, Yamamoto, H, Matsumoto, K, Kimura, T, Katsuragi, S, Miyakawa, T and Miyamoto, E (1999) Nuclear localization of the delta subunit of Ca2+/calmodulin-dependent protein kinase II in rat cerebellar granule cells. J. Neurochem. 72:815-25 |
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| Abstract |
To examine the physiological roles of the delta subunit of Ca2+/calmodulin-dependent protein kinase II (CaM kinase IIdelta) in brain, we examined the localization of CaM kinase IIdelta in the rat brain. A specific antibody to CaM kinase IIdelta1-delta4 isoforms was prepared by immunizing rabbits with a synthesized peptide corresponding to the unique carboxyl-terminal end of these isoforms. The prepared antibody did not recognize the alpha, beta, and gamma subunits, which were each overexpressed in NG108-15 cells. Immunoblot analysis on various regions and the nuclear fractions from rat brains suggested that some isoforms of CaM kinase IIdelta1-delta4 were abundant in the nucleus in the cerebellum. Total RNA from the cerebellum was analyzed by RT-PCR with a primer pair from variable domain 1 to variable domain 2. We detected the three PCR products delta3.1, delta3.4, and delta3 that contained the nuclear localization signal. These CaM kinase IIdelta3 isoforms were localized in the nuclei in transfected NG108-15 cells. Immunohistochemical study suggested the existence of these isoforms in the nuclei in cerebellar granule cells. These results suggest that CaM kinase IIdelta3 isoforms are involved in nuclear Ca2+ signaling in cerebellar granule cells. |
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| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Calcium-Calmodulin-Dependent Protein Kinase Type 2; Calcium-Calmodulin-Dependent Protein Kinases/analysis; Calcium-Calmodulin-Dependent Protein Kinases/chemistry; Calcium-Calmodulin-Dependent Protein Kinases/genetics; Cell Nucleus/enzymology; Cerebellum/cytology; Cerebellum/enzymology; Gene Expression Regulation, Enzymologic; Glioma; Immunoblotting; Molecular Sequence Data; Neurons/cytology; Neurons/enzymology; Nuclear Localization Signals/physiology; Rabbits; Rats; Subcellular Fractions/enzymology; Transfection; Tumor Cells, Cultured/enzymology |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0005634: nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
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