PMID:9576906

Citation	Hogenesch, JB, Gu, YZ, Jain, S and Bradfield, CA (1998) The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors. Proc. Natl. Acad. Sci. U.S.A. 95:5474-9
Abstract	We report that MOP3 is a general dimerization partner for a subset of the basic-helix-loop-helix (bHLH)-PER-ARNT-SIM (PAS) superfamily of transcriptional regulators. We demonstrated that MOP3 interacts with MOP4, CLOCK, hypoxia-inducible factor 1alpha (HIF1alpha), and HIF2alpha. A DNA selection protocol revealed that the MOP3-MOP4 heterodimer bound a CACGTGA-containing DNA element. Transient transfection experiments demonstrated that the MOP3-MOP4 and MOP3-CLOCK complexes bound this element in COS-1 cells and drove transcription from a linked luciferase reporter gene. We also deduced the high-affinity DNA binding sites for MOP3-HIF1alpha complex (TACGTGA) and used transient transfection experiments to demonstrate that the MOP3-HIF1alpha and MOP3-HIF2alpha heterodimers bound this element, drove transcription, and responded to cellular hypoxia. Finally, we found that MOP3 mRNA expression overlaps in a number of tissues with each of its four potential partner molecules in vivo.
Links	PubMed PMC20401
Keywords	Animals; Basic Helix-Loop-Helix Transcription Factors; CLOCK Proteins; COS Cells; Circadian Rhythm; DNA/metabolism; DNA-Binding Proteins/metabolism; Dimerization; Helix-Loop-Helix Motifs; Hypoxia-Inducible Factor 1; Hypoxia-Inducible Factor 1, alpha Subunit; Nuclear Proteins/metabolism; Protein Binding; Trans-Activators/metabolism; Transcription Factors; Transfection
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
HUMAN:HIF1A	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:O00327	P		Seeded From UniProt	complete
HUMAN:HIF1A	part_of	GO:0005667: transcription factor complex	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O00327	C		Seeded From UniProt	complete
HUMAN:BMAL1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q99814	P		Seeded From UniProt	complete
HUMAN:BMAL1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q99743	P		Seeded From UniProt	complete
HUMAN:BMAL1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q16665	P		Seeded From UniProt	complete
HUMAN:BMAL1	involved_in	GO:0045944: positive regulation of transcription by RNA polymerase II	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:O15516	P		Seeded From UniProt	complete
HUMAN:BMAL1	part_of	GO:0005667: transcription factor complex	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q99814	C		Seeded From UniProt	complete
HUMAN:BMAL1	part_of	GO:0005667: transcription factor complex	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q99743	C		Seeded From UniProt	complete
HUMAN:BMAL1	part_of	GO:0005667: transcription factor complex	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:Q16665	C		Seeded From UniProt	complete
HUMAN:BMAL1	part_of	GO:0005667: transcription factor complex	ECO:0000353: physical interaction evidence used in manual assertion	UniProtKB:O15516	C		Seeded From UniProt	complete
HUMAN:BMAL1	enables	GO:0003677: DNA binding	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:Q99743	F		Seeded From UniProt	complete

References

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