GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9287030
| Citation |
Daniel, RA, Haiech, J, Denizot, F and Errington, J (1997) Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR. J. Bacteriol. 179:5636-8 |
|---|---|
| Abstract |
We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli. New strains with insertions in the lacA gene should be of use in studies using lacZ fusions in B. subtilis. |
| Links | |
| Keywords |
Bacillus subtilis/enzymology; Bacillus subtilis/genetics; Cloning, Molecular; DNA Transposable Elements; Genes, Bacterial/genetics; Genes, Regulator/genetics; Mutagenesis, Insertional; Repressor Proteins/genetics; Restriction Mapping; Sequence Analysis, DNA; Sequence Homology, Amino Acid; beta-Galactosidase/biosynthesis; beta-Galactosidase/genetics |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0004565: beta-galactosidase activity |
ECO:0000315: |
F |
LacR is known to be a regulator for lacA activity. Figure 2 shows that when mutant lacR strains are utilized, beta galactosidase activity is up regulated as long as there are no mutations in lacA. Mutant lacA strains results in decreased or no beta galactosidase activity showing that it is necessary for it to occur. |
complete | ||||
| GO:0006355: regulation of transcription, DNA-dependent |
ECO:0000315: |
P |
Table 2 shows that mutant lacR strains upregulated beta-galactosidase activity within the bacteria provided they were combined with wild type lacA strains. The mutant colonies were reported as expressing a blue color along with the increased activity. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
